Skip to main content Skip to main navigation menu Skip to site footer
Original article
Austral J. Vet. Sci.
Vol 53, 99-108 (2021)
DOI: 10.4067/S0719-81322021000200099

Aquatic pollution from anthropogenic discharges modulates gene expression in liver of rainbow trout (Oncorhynchus mykiss)

1 Instituto Bioquímica y Microbiología, Facultad Ciencias, Universidad Austral de Chile, Valdivia, Chile.
2 Escuela de Graduados, Programa de Doctorado en Ciencias de la Acuicultura, Universidad Austral de Chile, Puerto Montt, Chile; Instituto Ciencias Marinas y Limnologicas, Facultad Ciencias, Universidad Austral de Chile, Valdivia, Chile.
3 Bavarian Environment Agency, Unit Aquatic Ecotoxicology, Microbial Ecology, Wielenbach, Germany
4 Instituto Ciencias Marinas y Limnologicas, Facultad Ciencias, Universidad Austral de Chile, Valdivia, Chile; Centro Fondap de Investigación de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile.
Keywords: anthropogenic discharges in river gene expression liver biomarkers environmental exposure Oncorhynchus mykiss

Submitted: 2020-11-10

Accepted: 2021-01-26

Published: 2021-05-01

How to Cite

Valenzuela-Nieto, G. E., Leal, C., Schwaiger, J., Ferling, H., Vargas-Chacoff, L., & Kausel, G. (2021). Aquatic pollution from anthropogenic discharges modulates gene expression in liver of rainbow trout (Oncorhynchus mykiss). Austral Journal of Veterinary Sciences, 53(2), 99–108. https://doi.org/10.4067/S0719-81322021000200099

License

Copyright (c) 2021 Guillermo E. Valenzuela-Nieto, Carlos Leal, Julia Schwaiger, Hermann Ferling, Luis Vargas-Chacoff, Gudrun Kausel

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

With the aim to characterise the biological impact of anthropogenic discharge in a river, gene expression in fish was evaluated as a biomarker for mixture effects of potential toxic compounds in the aquatic environment. Adult male rainbow trout (Oncorhynchus mykiss) were used as monitoring organisms. Within the German experimental set up, trout were exposed actively for 28 days in biologically purified sewage as well as in river water up- and downstream of the sewage treatment plant (STP) effluent. In a different approach, wild trout were captured in southern Chile near anthropogenic discharges. Fish were taken from river Maullin receiving sewage from a settlement and from river Pescado influenced by effluents from a fish farm. In vivo effects were assessed by RT-qPCR analyses of biomarker gene expression, vitellogenin (vg), metallothionein (mt) and cytochrome 1A (cyp1A) in liver samples with primers amplifying specific sequences previously confirmed by cloning and sequencing. The modulation of expression of marker genes involved in metal stress, reproduction and detoxifying systems in the liver of male rainbow trout revealed organismal response to anthropogenic contamination in two different study areas, such as Chile and Germany, thereby indicating a potential risk on the aquatic ecosystems.

Downloads

Download data is not yet available.

References

  1. Alonso Á, Figueroa R, Castro-Díez P. 2017. Pollution assessment of the Biobío River (Chile): Prioritization of substances of concern under an ecotoxicological approach. Environ Manage 59, 856-869.
  2. Arukwe A. 2002. Complementary DNA cloning, sequence analysis and differential organ expression of β-naphthoflavone-inducible cytochrome P4501A in Atlantic salmon (Salmo salar). Comp Biochem Physiol - C Toxicol Pharmacol 133, 613-624.
  3. Benninghoff A, Williams D. 2008. Identification of a transcriptional fingerprint of estrogen exposure in rainbow trout liver. Toxicol Sci 101, 65-80.
  4. Buhler D, Wang-Buhler J.L. 1998. Rainbow trout cytochrome P450s: purification, molecular aspects, metabolic activity, induction and role in environmental monitoring. Comp Biochem Physiol - C Pharmacol Toxicol Endocrinol 121, 107-137.
  5. Burkina V, Rasmussen M, Pilipenko N, Zamaratskaia G. 2017. Comparison of xenobiotic-metabolising human, porcine, rodent, and piscine cytochrome P450. Toxicology 375, 10-27.
  6. Cárcamo JG, Aguilar M, Carreño C, Vera T, Arias-Darraz L, et al. 2017. Consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol Part - C Toxicol Pharmacol 191, 129-137.
  7. Chen W, Christopher-John J, Lin C, Chang C. 2007. Expression pattern of metallothionein, MTF-1 nuclear translocation, and its DNA-binding activity in zebrafish (Danio rerio) induced by zinc and cadmium. Environ Toxicol Chem 26, 110-117.
  8. Chiang G, Barra R, Díaz-Jaramillo M, Rivas M, Bahamonde P, et al. 2015. Estrogenicity and intersex in juvenile rainbow trout (Oncorhynchus mykiss) exposed to Pine/Eucalyptus pulp and paper production effluent in Chile. Aqua Toxicol 164, 126-134.
  9. Cocherell SA, Jones GJ, Miranda JB, Cocherell DE, Cech JJ, et al. 2010. Distribution and movement of domestic rainbow trout, Oncorhynchus mykiss, during pulsed flows in the South Fork American River, California. Environ Biol Fish 89, 105-116.
  10. Connon R, D’Abronzo L, Hostetter N, Javidmehr A, Roby D, et al. 2012. Transcription profiling in environmental diagnostics: Health assessments in Columbia River basin steelhead (Oncorhynchus mykiss). Environ Sci Technol 46, 6081-6087.
  11. Copeland P, Sumpter J, Walker T, Croft M. 1986. Vitellogenin levels in male and female rainbow trout (Salmo gairdneri Richardson) at various stages of the reproductive cycle. Comp Biochem Physiol - B Biochem Mol Biol 83, 487-493.
  12. Dedual M, Jowett IG. 1999. Movement of rainbow trout (Oncorhynchus mykiss) during the spawning migration in the Tongariro River, New Zealand. N Z J Mar Freshwater Res 33, 107-117.
  13. Gagné F, Smyth S, André C, Douville M, Gélinas M, et al. 2013. Stressrelated gene expression changes in rainbow trout hepatocytes exposed to various municipal wastewater treatment influents and effluents. Environ Sci Pollut Res 20, 1706-1718.
  14. Habera JW, Kulp MA, Moore SE. 2010. Three-pass depletion sampling accuracy of two electric fields for estimating trout abundance in a low-conductivity stream with limited habitat complexity. N Am J Fish Manag 40, 757-766.
  15. Hamilton P, Nicol E, De-Bastos E. Williams R, Sumpter J, et al. 2014. Populations of a cyprinid fish are self-sustaining despite widespread feminization of males. BMC Biol 12, 1. 108
  16. VALENZUELA-NIETO ET AL He Y, Folkerts E, Zhang Y, Martin J, Alessi D, et al. 2017. Effects on biotransformation, oxidative stress, and endocrine disruption in rainbow trout (Oncorhynchus mykiss) exposed to hydraulic fracturing flowback and produced water. Environ Sci Technol 51, 940-947.
  17. Henneberg A, Triebskorn R. 2015. Efficiency of advanced wastewater treatment technologies for the reduction of hormonal activity in effluents and connected surface water bodies by means of vitellogenin analyses in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta f. fario). Environ Sci Eur 27, 1-12.
  18. Holmstrup M, Bindesbøl A, Oostingh G, Duschl A, Scheil V. 2010. Interactions between effects of environmental chemicals and natural stressors: A review. Sci Total Environ 408, 3746-3762.
  19. Ings J, Servos M, Vijayan M. 2011. Exposure to municipal wastewater effluent impacts stress performance in rainbow trout. Aquat Toxicol 103, 85-91.
  20. Kagi J. 1993. Evolution, structure and chemical activity of class I metallothioneins: An overview. In: Suzuki KT, Imura N, Kimura M (eds). Metallothionein III, Biological Roles and Medical Implications. Birkhauser Verlag, Basel, Switzerland, Pp 29-55.
  21. Kidd K, Blanchfield P, Mills K, Palace V, Evans J, et al. 2007. Collapse of a fish population after exposure to a synthetic estrogen. Proc Natl Acad Sci USA 104, 8897-8901.
  22. Kortenkamp A. 2017. Endocrine disruptors: The burden of endocrinedisrupting chemicals in the USA. Nat Rev Endocrinol 13, 6-7.
  23. Kumar G, Denslow N. 2017. Gene expression profiling in fish toxicology: A Review. Rev Environ Contam Toxicol 241, 1-38.
  24. Livak K, Schmittgen T. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔct Method. Methods 25, 402-408.
  25. Marlatt V, Sherrard R, Kennedy C, Elphick J, Martyniuk C. 2016. Application of molecular endpoints in early life stage salmonid environmental biomonitoring. Aquat Toxicol 173, 178-191.
  26. Martyniuk CJ, Feswick A, Munkittrick KR, Dreier DA, Denslow ND. 2020. Twenty years of transcriptomics, 17alpha-ethinylestradiol, and fish. Gen Comp Endocrinol 286 113325.
  27. Mommsen T, Walsh P. 1988. Vitellogenesis and oocyte assembly. In: Hoar WS, Randall DJ (eds). Fish Physiology.Vol XIA. Academic Press, New York, USA, 347-406.
  28. Nimptsch J, Woelfl S, Osorio S, Valenzuela J, Ebersbach, et al. 2015. Tracing dissolved organic matter (DOM) from land-based aquaculture systems in North Patagonian streams. Sci Total Environ 537, 129-138.
  29. Osachoff H, Brown L, Tirrul L, Van Aggelen G, Brinkman F, et al. 2016. Time course of hepatic gene expression and plasma vitellogenin protein concentrations in estrone-exposed juvenile rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol - Part D Genomics Proteomics 19, 112-119.
  30. Pfaffl M. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29, 2003-2007.
  31. Pottier G, Beaumont WR, Marchand F, Le Bail P-Y, Azam D, et al. 2019. Electrofishing in streams of low water conductivity but high biodiversity value: Challenges, limits and perspectives. Fish Manag Ecol 27, 53-63.
  32. Rappaport S, Smith M. 2010. Environment and disease risks. Science 330, 460-461. Ricketts C, Bates W, Reid S. 2015. The effects of acute waterborne exposure to sublethal concentrations of molybdenum on the stress response in rainbow trout, Oncorhynchus mykiss. PLoS One 10, 1-22.
  33. Roesijadi G. 1994. Metallothionein induction as a measure of response to metal exposure in aquatic animals. Environ Health Perspect 102, 91-95.
  34. Sambrook J, Fritsch E, Maniatis T. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, New York, USA.
  35. Schwaiger J, Negele R. 1998. Plasma vitellogenin - A blood parameter to evaluate exposure of fish to xenoestrogens. Acta Vet Brno 67, 257-264.
  36. Tattam IA, Li HW, Giannico GR, Ruzycki JR. 2016. Seasonal changes in spatial patterns of Oncorhynchus mykiss growth require year-round monitoring. Eco Freshw Fish 26 434-443.
  37. Triebskorn R, Adam S, Casper H, Honnen W, Pawert M. 2002. Biomarkers as diagnostic tools for evaluating effects of unknown past water quality conditions on stream organisms. Ecotoxicology 11, 451-465.
  38. Tyler C, Jobling S, Sumpter J. 1998. Endocrine disruption in wildlife: A critical review of the evidence. Crit Rev Toxicol 28, 319-361.
  39. Valenzuela G, Perez A, Navarro M, Romero A, Figueroa J, et al. 2015. Differential response of two somatolactin genes to zinc or estrogen in pituitary of Cyprinus carpio. Gen Comp Endocrinol 215, 98-105.
  40. Wilson J, Berntsen H, Zimmer K, Verhaegen S, Frizzella C, et al. 2016. Do persistent organic pollutants interact with the stress response? Individual compounds, and their mixtures, interaction with the glucocorticoid receptor. Toxicol Lett 241, 121-132.
  41. Zhou H, Wu H, Liao C, Diao X, Zhen J, et al. 2010. Toxicology mechanism of the persistent organic pollutants (POPs) in fish through AhR pathway. Toxicol Mech Methods 20, 279-286.

Make a Submission

Make a Submission

Journal Metrics (2023) & Ranking

Impact Factor
→
0.5 (2024)
5 years Impact Factor
→
0.8
JCR Quartile
→
Q4
JIF Rank
→
134/170 (Veterinary Sciences)
SJR (2024)
→
0.244
SNIP (2024)
→
0.35

 

 

SCImago Journal & Country Rank

Indexed in

Publisher


This journal is published by the Facultad de Ciencias Veterinarias, Universidad Austral de Chile

Keywords