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Original article
Austral J. Vet. Sci.
Vol 50, 35-41 (2018)
DOI: 10.4067/S0719-81322018000100107%20

Probiotics on performance, intestinal morphology and carcass characteristics of broiler chickens raised with lower or higher environmental challenge

1 University of Western São Paulo, São Paulo, Brazil.
2 University of West Santa Catarina, Florianópolis, Brazil.
3 São Paulo State University, College of Agricultural and Technological Sciences, São Paulo, Brazil.
4 Department of Animal Nutrition and Production, University of São Paulo, São Paulo, Brazil.
Keywords: additives intestinal health poultry sanitary challenge

Submitted: 2017-05-19

Accepted: 2017-08-29

Published: 2018-01-01

*Corresponding author:
mfcb@usp.br

How to Cite

de Souza, L. F., Araújo, D. N., Stefani, L. M., Giometti, I. C., Cruz-Polycarpo, V. C., Polycarpo, G., & Burbarelli, M. F. (2018). Probiotics on performance, intestinal morphology and carcass characteristics of broiler chickens raised with lower or higher environmental challenge. Austral Journal of Veterinary Sciences, 50(1), 35–41. https://doi.org/10.4067/S0719-81322018000100107

License

Copyright (c) 2018 Lilian F.A. de Souza, Denise N. Araújo, Lenita M. Stefani, Ines C. Giometti, Valquíria C. Cruz-Polycarpo, Gustavo Polycarpo, Maria F. Burbarelli

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

This study aimed to evaluate the effect of probiotics on performance, intestinal morphology and carcass characteristics of broiler chickens housed on lower or higher environmental challenge. Three hundred male Cobb chicks were distributed into four groups in completely randomised design with treatments arranged in a 2 × 2 factorial scheme to evaluate effects of two diets (with or without probiotics) and two environmental conditions (lower or higher challenge), totaling four treatments with five replications with 15 birds per box. Probiotics were added on diets and were composed of Lactobacillus acidophilus, Bacillus subtilis, Bifidobacterium bifidum and Enterococcus faecium. The environment with lower challenge was made up of new wood shavings used as litter, low bird density (8 birds/m2) and daily-cleaned bell drinkers. The environment with higher challenge was made up of re-used wood shavings used as litter (after three broods of broilers), bird density of 16 birds/m2 with bell drinkers cleaned every two days. No significant interaction between diet and environmental challenge was found for any of the variables evaluated. Performance, slaughterhouse variables and chemical carcass composition were not affected by the use of probiotics. Chicks receiving diets without probiotics had lower intestinal crypt depth (267.1 vs. 316.6 ?m, P=0.0068). Birds raised in the environment with higher challenge decreased feed intake (4,660 vs 5,020 g, P=0.0422), weight gain (2,610 vs 2,810 g, P= 0.0054), drumstick and thigh yield (21.98 vs 24.14 %, P=0.0354), and increased crypt depth (325.2 vs 258.5 μm P=0.0009). In conclusion, the probiotic does not promote satisfactory improvements, regardless of the environmental challenge.

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References

  1. Applegate TJ, Kitchell ML, Uni Z, Lilburn MS. 1999. Intestinal villus growth, enterocyte migration and proliferation of the turkey poultry. Comp Biochem Physi 124, 371-380.
  2. Baracho MS, Cassiano JA, Nääs IA, Tonon GS, Garcia RG, et al. 2013. Ambiente interno em galpões de frango de corte com cama nova e reutilizada. Agrarian 6, 473-478.
  3. Barton MD. 2000. Antibiotic use in animal feed and its impact on human health. Nutr Res Rev 13, 279-299.
  4. Beloor J, Kang HK, Kim YJ, Subramani VK, Jang IS, et al. 2010. The effect of stocking density on stress related genes and telomeric length in broiler chickens. Asian-Austral J Anim 23, 437-443.
  5. Boostani A, Mahmoodian Fard HR, Ashayerizadeh A, Aminafshar M. 2013. Growth performance, carcass yield and intestinal microflora populations of broilers fed diets containing thepax and yogurt. Rev Bras Cienc Avíc 15, 1-6.
  6. Chinivasagam, HN, Redding M, Runge G, Blackall PJ. 2010. Presence and incidence of food-borne pathogens in Australian chicken litter. Brit Poultry Sci 51, 311-318.
  7. Costa CAF, Guidoni AL, Paiva DP, Ávila VS. 2000. Coccidiosis and performance in broilers with anticoccidial medicated feed starting at different ages. Arq Bras Med Vet e Zoot 52, 144-149.
  8. Cressman MD, Yu Z, Nelson MC, Moeller SJ, Lilburn MS, et al. 2010. Interrelations between the microbiotas in the litter and in the intestines of commercial broiler chickens. Appl Environ Microb 76, 6572-6582.
  9. Domingues CH, Santos ET, Castiblanco DC, de Quadros TCO, Petrolli TG, et al. 2014. Avaliação do desempenho e rendimento de carcaça de frangos de corte alimentados com dietas contendo probiótico nas diferentes fases de criação. Rev Agrocientífica 1, 7-16.
  10. Dozier WA, Thaxton JP, Branton SL, Morgan GW, Miles DM, et al. 2005. Stocking density effects on growth performance and processing yields of heavy broilers. Poult Sci 84, 1332-1338.
  11. Dozier WA, Thaxton JP, Purswell JL, Olanrewaju HA, Branton SL, et al. 2006. Stocking density effects on male broilers grown to 1.8 kilograms of body weight. Poult Sci 85, 344-351.
  12. Fallah R, Saghafi M, Rezaei H, Parvar R. 2013 Effect of Bioplus 2B® and Protoxin probiotics supplementation on growth performance, small intestinal morphology and carcass characteristics of broiler chickens Brit J Poult Sci 2, 11-15.
  13. Forbes M, Park JT. 1959. Growth of germ-free and conventional chicks. Effect of diet, dietary penicillin and bacterial environment. J Nutr 67, 69-84.
  14. Fuller R. 1989. Probiotics in man and animals. J Appl Bact 66, 365-378.
  15. Furlan LR, Macari M, Luquetti BC. 2004. Como avaliar os efeitos do uso de prebióticos, probióticos e flora de exclusão competitiva. In: Simpósio técnico de incubação, matrizes de corte e nutrição, Balneário Camboriú. Anais 6-28.
  16. Garcia RG, Mendes AA, Garcia EA, Nääs IA, Moreira J, et al. 2002. Efeito da densidade de criação e do sexo sobre o empenamento, incidência de lesões na carcaça e qualidade da carne de peito de frangos de corte. Rev Bras Cienc Avíc 4, 1-9.
  17. Giannenas I, Tontis D, Tsalie E, Chronis EF, Doukas D, et al. 2010. Influence of dietary mushroom agaricus bisporus on intestinal morphology and microflora composition in broiler chickens. Res Vet Sci 89, 78-84.
  18. Guardia S, Konsak B, Combes S, Levenez F, Cauquil L, et al. 2011. Effects of stocking density on the growth performance and digestive microbiota of broiler chickens. Poult sci 90, 1878-1889.
  19. Gunal M, Yayli G, Kaya O, Karahan N, Sulak O. 2006. The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. Int J Poult Sci 5, 149-155.
  20. Hess JB, Macklin KS, Bilgili SF, Blake JP, Norton RA. 2008. In-house litter composting between flocks. In: Proceedings 2008 National Poultry Waste Management Symposium, October 21-23, Des Moines, Iowa, USA.
  21. Huyghebaert G, Ducatelle R, Van Immerseel F. 2011. An update on alternatives to antimicrobial growth promoters for broilers. Vet J 187, 182-188.
  22. Jankowski J, Mikulski D, Tatara MR, Krupski W. 2014. Effects of increased stocking density and heat stress on growth, performance, carcase characteristics and skeletal properties in turkeys. Vet Rec, doi: 10.1136/vr.102216.
  23. Jin LZ, Ho HW, Abdullah N, Jalaludin S. 2000. Digestive and bacteria enzyme activities in broilers fed diets supplemented with Lactobacillus cultures. Poul Sci 79, 886-891.
  24. Lee KW, Lillehoj HS, Lee SH, Jang SI, Ritter GD, et al. 2011. Impact of fresh or used litter on the posthatch immune system of commercial broilers. Avian Dis 55, 539-544.
  25. Malone B. 2006. Managing built-up litter. In: Proceedings to 2006 Midwest Poultry Federation Conference, St. Paul, MN, USA
  26. Marubashi T, Gracia MI, Esteve-Garcia E, Piskoríková M. 2012. The efficacy of the probiotic feed additive Calsporin® (Bacillus subtilis C-3102) in broilers: combined analysis of four different studies. J Appl Anim Nut 1, 878-901.
  27. Maiorka A, Macari M. 2002. Absorção de minerais. In: Macari M, Furlan RL, Gonzales E (eds). Fisiologia aviária aplicada a frangos de corte. FUNEP/UNESP, Jaboticabal , Brazil.
  28. Mendes AAA, Nääs IA, Macari M. 2004. Produção de frangos de corte. 1ª ed. Facta, SP, Brazil. Muniz E, Mesa D, Cuaspa R, Souza AM, Santin E. 2014. Presence of Salmonella spp. in reused broiler litter. Rev Colomb Cienc Pec 27, 12-27.
  29. Mountzouris KC, Tsirtsikos P, Palamidi I, Arvaniti A, Mohnl M, et al. 2010. Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and caecal microflora composition. Poult Sci 89, 58-67.
  30. Nosrati M, Javandel F, Camacho LM, Khusro A, Cipriano M, et al. 2017. The effects of antibiotic, probiotic, organic acid, vitamin C, and Echinacea purpurea extract on performance, carcass characteristics, blood chemistry, microbiota, and immunity of broiler chickens. J Appl Poult Res 26, 295-306.
  31. Oliveira MC, Ferreira HA, Cancherini LC. 2004. Efeito de condicionadores químicos sobre a qualidade da cama de frango. Arq Bras Med Vet Zootec 56, 536-541.
  32. Patel SG, Raval AP, Bhagwat SR, Sadrasaniya DA, Patel AP, et al. 2015. Effects of probiotics supplementation on growth performance, feed conversion ratio and economics of broilers. J Anim Res 5,155-160.
  33. Patterson JA, Burkholder KM. 2003. Application of prebiotics and probiotics in poultry production. Poult Sci 82, 627-631.
  34. Paryad A, Mahmoudi M. 2008. Effect of different levels of supplemental yeast (Saccharomyces cerevisiae) on performance, blood constituents and carcass characteristics of broiler chicks. African J Agri Res 3, 835-842.
  35. Pourakbari M, Seidavi A, Asadpour L, Martínez A. 2016. Probiotic level effects on growth performance, carcass traits, blood parameters, cecal microbiota, and immune response of broilers. Anais da Academia Brasileira de Ciências 88, 1011-1021.
  36. Puupponen-Pimiä R, Aura AM, Oksmancaldentey KM, Myllärinen P, Saarela M, et al. 2002. Development of functional ingredients for gut health. Trends Food Sci Tech, 13, 3-11.
  37. Ramos LSN, Lopes JB, Ribeiro MN, Silva FES, Merval RR, et al. 2014. Aditivos alternativos a antibióticos para frangos de corte no período de 22 a 42 dias de idade. Rev Bras Saúde Prod Anim 15, 897-906.
  38. Ravindran VDV, Thomas DG, Morel PCH. 2006. Performance and welfare of broilers as affected by stocking density and zinc bacitracin supplementation. J Anim Sci 77, 110-116.
  39. Rostagno HS, Albino LFT, Donzele JL, Gomes PC, Oliveira RF, et al. 2005. Composição de alimentos e exigências nutricionais. Tabelas brasileiras para aves e suínos. 2ª ed. Viçosa, MG, Brazil.
  40. Roll VFB, Dai Pra MA, Roll AP.2011. Research on Salmonella in broiler litter reused for up to 14 consecutive flocks. Poult Sci 90, 2257-2262.
  41. Samanya M, Yamauchi K. 2002. Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Comp Biochem Physiol 133, 95-104.
  42. SAS, Statistical Analysis System. 2004. SAS Version 9.1. SAS Institute Inc, Cary, NC, USA.
  43. Schneitz C, Mead G. 2000. Competitive exclusion. In: Wray C, Wray A (eds). Salmonella in domestic animals. CAB International, Wallingford, UK.
  44. Sen S, Ingale SL, Kim YW, Kim JS, Kim KH, et al. 2012. Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Res Vet Sci 93, 264-268.
  45. Shargh MS, Dastar B, Zerehdaran S, Khomeiri M, Moradi A. 2012. Effects of using plant extracts and a probiotic on performance, intestinal morphology, and microflora population in broilers. J Appl Poult Res 21, 201-208.
  46. Shim YH, Shinde PL, Choi JY, Kim JS, Seo DK, et al. 2010. Evaluation of multi-microbial probiotics produced by submerged liquid and solid substrate fermentation methods in broilers. Asian Austral J Anim Sci 23, 521-529.
  47. Silva DJ, Queiroz AC. 2002. Análise de alimentos: métodos químicos e biológicos. 3a ed. Editora UFV, Viçosa, MG, Brazil.
  48. Souza LFAD, Araujo DN, Astolphi JLDL, Ambiel AC, Santos LS, et al. 2011. Probiótico e antibiótico como promotores de crescimento para frangos de corte. Colloq Agr 6, 33-39.
  49. Timmerman HM, Veldman A, van den Elsen E, Rombouts FM, Beynen AC. 2006. Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics. Poult Sci 85, 1383-1388.
  50. Uni Z, Ganot S, Sklan D. 1998. Posthatch development of mucosal function in the broiler small intestine. Poult Sci 77, 75-82.
  51. Zhou X, Wang Y, Gu Q, Li W. 2010. Effect of dietary probiotic, Bacillus coagulans, on growth performance, chemical composition, and meat quality of Guangxi Yellow chicken. Poult Sci 89, 588-593.

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