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Original article
Austral J. Vet. Sci.
Vol 49, 99-104 (2017)
DOI: 10.4067/S0719-81322017000200099%20

Cell block sensitivity for immunohistochemical detection of cytokeratin 5, oestrogen and progesterone receptors in canine primary mammary carcinoma

1 Department of Pathology, Botucatu Medical School, Universidade Estadual Paulista, São Paulo, Brazil.
2 Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista, São Paulo, Brazil.
3 Department of Pathology, Botucatu Medical School, Universidade Estadual Paulista, São Paulo, Brazil; Department of Pathology, Barretos Cancer Hospital and Faculty of Health Sciences Barretos Dr Paulo Prata, Barretos, Brazil.
4 Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista, São Paulo, Brazil; Veterinary Pathology Research Group, Faculty of Agricultural Sciences, Universidad de Caldas, Manizales, Colombia; GIDIMEVETZ, Universidad Pedagógica y Tecnológica de Colombia, Tunja-Boyacá, Colombia.
5 Department of Pathology, Botucatu Medical School, Universidade Estadual Paulista, São Paulo, Brazil; Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista, São Paulo, Brazil.
Keywords: cytoinclusion histopathological immunohistochemistry tumor

Submitted: 2016-08-15

Accepted: 2017-01-05

Published: 2017-05-01

*Corresponding author:
maomontoya53@yahoo.es

How to Cite

Silveira, T. L., Campos, L. M., Dufloth, R. M., Miot, H. A., Fêo, H. B., Montoya, L. M., & Rocha, N. S. (2017). Cell block sensitivity for immunohistochemical detection of cytokeratin 5, oestrogen and progesterone receptors in canine primary mammary carcinoma. Austral Journal of Veterinary Sciences, 49(2), 99–104. https://doi.org/10.4067/S0719-81322017000200099

License

Copyright (c) 2017 Tatiane L. Silveira, Luciana M. Campos, Rozany M. Dufloth, Helio A. Miot, Haline B. Fêo, Luis M. Montoya, Noeme S. Rocha

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Mammary carcinomas are relatively common ailments among female canines aged around 10 years old, presenting an important morbidity with an average survival of five years. The cytoinclusion technique is frequently employed in human medicine as the investigative method of choice as it quickly provides resources for the determination of the correct therapeutic response, however, the effectiveness of the technique in canines remains understudied in veterinary medicine. This study aims at evaluating the degree of correlation with immunohistochemical marking for cytokeratin 5 (CK5), oestrogen and progesterone receptors (ER and PR) between the cytoinclusion and the histopathology technique in mammary carcinomas. Twenty-five samples of mammary carcinoma, both for the cytoinclusion and histopathological techniques were submitted for histological processing; microscope slides were created for hematoxylin-eosin (HE) staining and the immunohistochemical technique (IHC) was assessed for the ER, PR and CK5 receptors. Through the HE staining, we reached a concordance rate of 100% between the cytoinclusion and the histopathological analysis in the diagnosis of carcinomas. The immunohistochemical assay presented sensitivity of 85.71%, 95.45% and 100% and Cohen’s kappa of 0.78, 0.84 and 0.95 for ER, PR and CK5, respectively, as well as 100% specificity and P<0.01 for all three markers. Therefore, cytoinclusion represents an accessible, fast and low-cost method, offering high sensitivity for the cytomorphological and immunohistochemical diagnosis of mammary carcinoma in female canines.

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This journal is published by the Facultad de Ciencias Veterinarias, Universidad Austral de Chile

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