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Austral J. Vet. Sci.
Vol 49, 199-203 (2017)
DOI: 10.4067/S0719-81322017000300199

Identification of non-tuberculous mycobacteria isolated from opossum (Didelphis virginiana) lymph nodes and characterisation of lesions

1 Centro Universitario de Investigación y Desarrollo Agropecuario (CUIDA), Universidad de Colima, Colima, México.
2 Facultad de Medicina Veterinaria y Zootecnia de la Universidad de Colima, Colima, México.
3 Departamento de Patología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Autónoma de Nuevo León, Nuevo León, México.
4 Departamento de Patología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Autónoma de Tamaulipas, Tamaulipas, México.
5 Department of Pathology and Microbiology Atlantic Veterinary College, University of Prince Edward Island, Prince Edward Island, Canada.
6 Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Keywords: mycobacteria tuberculosis opossums lesions

Submitted: 2016-06-06

Accepted: 2017-05-03

Published: 2017-09-01

*Corresponding author:
ljgm_cmv@hotmail.com

How to Cite

García, L. J., González, D. A., Prado, O. F., Macedo, J. R., Ramírez, R., Martínez, J., Barrios, H. B., López, A., & Constantino, F. (2017). Identification of non-tuberculous mycobacteria isolated from opossum (Didelphis virginiana) lymph nodes and characterisation of lesions. Austral Journal of Veterinary Sciences, 49(3), 199–203. https://doi.org/10.4067/S0719-81322017000300199

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Copyright (c) 2017 Luis J. García, David A. González, Omar F. Prado, Julio R. Macedo, R Ramírez, J Martínez, Hugo B. Barrios, A López, F Constantino

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The aim of this study was to investigate the presence of NTM in the lymph nodes of opossums (D. virginiana) and to characterise the microscopic changes in affected tissue. Retropharyngeal and tracheobronchial lymph nodes were collected postmortem from 18 opossums in the state of Colima, Mexico in 2013. The lymph nodes were also cultured for mycobacterial organisms and processed for histopathological examination. Bacteriological cultures yielded 5/18X100 (28%) isolates of NTM, which were subsequently identified as M. terrae, M. szulgai, M. gastri and M. asiaticum. Microscopic examination of the affected nodes revealed a necrotic granulomatous lymphadenitis (3/60%) composed of histiocytes, epithelioid cells and giant cells with intralesional alcoholresistant acid bacteria. An association between the sex of the opossum and the presence of NTM was observed. To our knowledge, this is the first report of NTM isolation in opossums with granulomatous lymphadenitis in Mexico.

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References

  1. Acosta R, Ocampo J, Arellano Z, Ramírez L, Martínez V, et al. 2012. Histopathological and PCR Mycobacterium bovis Identification in Mexican Opossums (Didelphis virginiana) from Hidalgo, Mexico. J Anim Vet Adv 11, 207-210.
  2. Bercovier H, Vincent V. 2001. Mycobacterial infections in domestic and wild animals due to Mycobacterium marinum, M. fortuitum, M. chelonae, M. porcinum, M. farcinogenes, M. smegmatis, M. scrofulaceum, M. xenopi, M. kansasii, M. simiae and M. genavense. Rev Sci Tech 20, 265-290.
  3. Casal M, Casal M. 2000. Las micobacterias atípicas como patógenos emergentes. Enf Em 2, 220-230.
  4. Cruz B, Ruiz L, Navarrete D, Espinoza E, Vázquez E, et al. 2014. Genetic diversity and relative abundance of Didelphis marsupialis and Didelphis virginiana in Chiapas, Mexico. Rev Mex Bio 85, 251-261.
  5. Gadkowski B, Stout E. 2008. Cavitary pulmonary disease. Clin Microbiol Rev 21, 305-33.
  6. García L, Vázquez J, Sarabia D, León V, García L, et al. 2010. Lesiones pulmonares en tlacuaches (Didelphis virginiana) infectados naturalmente por Paragonimus mexicanus en Colima, México. Vet Méx 41, 65-72.
  7. García ML, López RA, López A, Prado OF. 2012. Neumonía lipídica endógena en tlacuaches (Didelphis virginiana) capturados en el estado de Colima, México. Vet Mex 43, 241-246.
  8. García MP, García L. 2012. Infections due to rapidly growing mycobacteria. Enf Inf Microbiol Clin 30, 192-200.
  9. García WL, Fernández P, Gómez L, Benítez J, García A, et al. 2012. Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected Fallow deer (Dama dama). Vet Immunol Immunop 149, 66-75.
  10. García WL, Fernández P, Benítez J, Cerratos R, Cuesta J, et al. 2013. Reducing Eurasian wild boar (Sus scrofa) population density as a measure for bovine tuberculosis control: Effects in wild boar and sympatric fallow deer (Dama dama) population in Central Spain. Prev Vet Med 110, 435-446.
  11. García WL, Benítez J, Martínez R, Carranza J, Cerratos R, et al. 2015. Non-tuberculous mycobacteria in wild boar (Sus scrofa) from Southern Spain: epidemiological, clinical and diagnostic concerns. Transb Emerg Dis 62, 72-80.
  12. Gortázar C, Torres M, Acevedo P, Aznar J, Negro J, et al. 2011. Finetuning the space, time, and host distribution of mycobacteria in wildlife. BMC Microbiol 11, 1-16.
  13. Hall B, Bender C, Garner M. 2005. Mycobacteriosis in a black-tailed deer (Odocoileus hemionus columbianus) caused by Mycobacterium kansasii. J Zoo Wildlife Med 36, 115-116.
  14. Hernández JA. 2014. Identificación de micobacterias no tuberculosas aisladas de nódulos linfáticos de bovinos y caracterización de lesiones asociadas. Tesis Maestría en Ciencias Veterinarias y Zootécnicas, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, México.
  15. Holland M. 2001. Nontuberculous mycobacteria. Am J Med Sci 321, 49-55.
  16. Iseman D, Marras K. 2008. The importance of non-tuberculous mycobacterial lung disease. Am J Resp Crit Care Med 178, 999-1000.
  17. Jang S, Hirsh C. 2002. Rapidly growing members of the genus Mycobacterium affecting dogs and cats. J Am Anim Hosp Assoc 38, 217-220.
  18. Jaroso R, Vicente J, Martín MP, Aranaz A, Lyshchenko K, et al. 2010. Ante-mortem testing wild fallow deer for bovine tuberculosis. Vet Microbiol 146, 285-289.
  19. Koneman W, Winn C, Allen D, Janda M, Procop W, et al. 2008. Diagnóstico Microbiológico. Médica Panamericana. Buenos Aires, Argentina.
  20. Machackova M, Matlova L, Lamka J, Smolik J, Melicharek I, et al. 2003. Wild boar (Sus scrofa) as a possible vector of mycobacterial infections: review of literature and critical analysis of data from Central Europe between 1983 to 2001. Vet Med Czech 48, 51-65.
  21. Martín HMP, Höfle U, Vicente J, Ruiz F, Vidal D, et al. 2007. Lesions associated with Mycobacterium tuberculosis complex infection in the European wild boar. Tubercle 87, 360-367.
  22. Piersimoni C, Scarparo C. 2008. Pulmonary infections associated with non-tuberculous mycobacteria in immunocompetet patients. Lancet Infect Dis 8, 323-334.
  23. Proano F, Rigouts L, Brandt J, Dorny P, Ron J, et al. 2006. Preliminary observations on Mycobacterium spp. in dairy cattle in Ecuador. Am J Trop Med Hyg 75, 318-323.
  24. Prophet E, Mills B, Arrington J, Sobin H. 1995. Métodos Histotecnológicos. Washington DC: Instituto de Patología de las Fuerzas Armadas de los Estados Unidos de América (AFIP) y Registro de Patología de los Estados Unidos de América (ARP).
  25. Sato T, Shibuya H, Ohba S, Nojiri T, Shirai W. 2003. Mycobacteriosis in two captive Florida manatees (Trichechus manatus latirostris). J Zoo Wildlife Med 34, 184-188.
  26. Zanella G, Duvauchelle A, Hars J, Moutou F, Boschiroli ML, et al. 2008. Patterns of lesions of bovine tuberculosis in wild red deer and wild boar. Vet Rec 163, 43-47.

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