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Original article
Austral J. Vet. Sci.
Vol 58, e5803 (2026)
DOI: 10.4206/ajvs.58.3

Dietary supplementation of Moringa oleifera leaf extract enhances productivity, carcass yield, gut microbiota and biomarker profiles in broiler chickens

1 Guangzhou College of Technology and Business, Guangzhou, People’s Republic of China.
2 Department of Livestock and Production Management, Faculty of Veterinary Science, The University of Veterinary and Animal Science, Swat, Pakistan.
3 Agriculture Development Company-LTD, Riyadh, Saudi Arabia.
4 Department of Animal Production, College of Food and Agriculture, King Saud University, Riyadh, Saudi Arabia.
Keywords: Moringa oleifera broiler chicken growth performance carcass traits physiological biomarkers antioxidant activity

Submitted: 2025-10-13

Accepted: 2026-01-12

Published: 2026-03-13

*Corresponding author:
assaralishah@yahoo.com

How to Cite

Chen, Z., Shah, A. A., Abdul Razaq, A. H., & Alhidary, I. (2026). Dietary supplementation of Moringa oleifera leaf extract enhances productivity, carcass yield, gut microbiota and biomarker profiles in broiler chickens. Austral Journal of Veterinary Sciences, 58, e5803. https://doi.org/10.4206/ajvs.58.3

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Copyright (c) 2026 Zhaojie CHEN, Assar Ali Shah, Abdul Hafeez Abdul Razaq, Ibrahim Alhidary

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

 

Plant feed additives produced as herbs, spices, and botanical extracts have presented themselves as possible substitutes for antimicrobial growth promoters (AGPs). These agents are valued for their growth-stimulating activities, antimicrobial effects, antioxidative properties, and immunomodulatory effects. This study aimed to determine the effects of Moringa oleifera leaf extract (MOLE) on the growth performance, carcass attributes, and physiological indicators of broiler chickens. A total of 240 one-day-old broiler chicks were assigned to four treatments: a control (no MOLE) and three levels of dietary MOLE (100, 200, and 400 mg MOLE/kg feed) for a feeding period of 42 days. Growth performance characteristics, such as weight gain, feed conversion ratio (FCR), and feed intake per day, were measured. Carcass characteristics and blood samples were examined at the end of the trial for liver and kidney function biomarkers, antioxidant enzymes, and lipid profiles. The supplementation of MOLE in diet significantly increased weight gain and FCR, especially at the level of 200 mg/kg. The inclusion of MOLE has been observed to improve carcass yield, particularly in the breast and thigh muscles of broilers. Furthermore, the MOLE-supplemented groups exhibited lower serum cholesterol levels, increased activity of some antioxidant enzymes (SOD and GPx), and improved liver and kidney function markers. In conclusion, MOLE has great potential as a feed additive to increase the productivity and improve the physiological health of broilers.

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References

  1. Abbasi, I.A., Shamim, A., Shad, M.K., Ashari, H. & Yusuf, I. (2024). Circular economy-based integrated farming system for indigenous chicken: Fostering food security and sustainability. Journal of Cleaner Production, 436, 140368. https://doi.org/10.1016/j.jclepro.2023.140368
  2. Abdel-Raheem, S.M. & Hassan, E.H. (2021). Effects of dietary inclusion of Moringa oleifera leaf meal on nutrient digestibility, rumen fermentation, ruminal enzyme activities and growth performance of buffalo calves. Saudi Journal of Biological Sciences, 28(8), 4430-4436. https://doi.org/10.1016/j.sjbs.2021.04.037
  3. Abu Hafsa, S.H., Ibrahim, S.A., Eid, Y.Z. & Hassan, A.A. (2020). Effect of dietary Moringa oleifera leaves on the performance, ileal microbiota and antioxidative status of broiler chickens. Journal of animal physiology and animal nutrition, 104(2), 529-538. https://doi.org/10.1111/jpn.13281
  4. Ahmad, M., Rasheed, M.A., Sattar, A., Abbas, G. & Basharat, A. (2022, March 8-10). Impact of antibiotic growth promoters (AGPS) in poultry production and alternative strategies. [Paper presentation]. 1st International Conference on Sustainable Ecological Agriculture (1st ICSEA), Konya, Türkiye. http://first.icseac.com/pbook2022.pdf
  5. Alfifi, A., El-Hamid, M.I.A., Abdel-Raheem, S.M., Al-Khalaifah, H.S., Youssef, W., Khalil, S.S., Al-Nasser, A., Elkhawaga, E., Elmehrath, E.M., Nassar, A.H. & Elsaid, G.A. (2025). Combined modulatory effects of dietary arginine and olive leaf phenolic extract on growth performance and immune functions of broiler chickens, and meat antioxidant potential during frozen storage. BMC Veterinary Research, 21(1), 226. https://doi.org/10.1186/s12917-025-04663-6
  6. Attah, A.F., Akindele, O.O., Nnamani, P.O., Jonah, U.J., Sonibare, M.A. & Moody, J.O. (2022). Moringa oleifera seed at the interface of food and medicine: Effect of extracts on some reproductive parameters, hepatic and renal histology. Frontiers in Pharmacology, 13, 816498. https://doi.org/10.3389/fphar.2022.816498
  7. Awan, A.K., Sultana, N., Khan, R.A., Sultana, R., Sahibzada, F.A., Khan, R.U., Khan, N.U., Khan, N.A., Zaman, S.H., Shah, M.S. & Shah, A.A. (2024). Antioxidant and Antidiabetic Potentials of Punica granatum Peel Extracts in Alloxan-Induced Diabetic Albino Rats. Pakistan Journal of Zoology, 56(6), 2685-2694. https://dx.doi.org/10.17582/journal.pjz/20220515110519
  8. Ayssiwede, S.B., Dieng, A., Bello, H., Chrysostome, C.A.A.M., Hane, M.B., Mankor, A., Dahouda, M., Houinato, M.R., Hornick, J.L. and Missohou, A. (2011). Effects of Moringa oleifera (Lam.) leaves meal incorporation in diets on growth performances, carcass characteristics and economics results of growing indigenous Senegal chickens. Pakistan Journal of Nutrition, 10(12), 1132-1145. https://doi.org/10.3923/pjn.2011.1132.1145
  9. Bhat, T.K., Kannan, A., Singh, B. & Sharma, O.P. (2013). Value addition of feed and fodder by alleviating the antinutritional effects of tannins. Agricultural Research, 2(3), 189-206. https://doi.org/10.1007/s40003-013-0066-6
  10. Coello, K.E., Frias, J., Martínez-Villaluenga, C., Cartea, M.E., Abilleira, R. & Peñas, E. (2020). Potential of germination in selected conditions to improve the nutritional and bioactive properties of moringa (Moringa oleifera L.). Foods, 9(11), 1639. https://doi.org/10.3390/foods9111639
  11. Devkota, S. and Bhusal, K.K. (2020). Moringa oleifera: a miracle multipurpose tree for agroforestry and climate change mitigation from the Himalayas–a review. Cogent Food & Agriculture, 6, 1805951. https://doi.org/10.1080/23311932.2020.1805951
  12. Elahwl, E.A., Assar, D.H., Al-Hawary, I.I., Salah, A.S., Ragab, A.E., Elsheshtawy, A., Assas, M., Abo-Al-Ela, H.G., Fouad, A.M. & Elbialy, Z.I. (2025). Alleviation of glyphosate-induced toxicity by Horseradish tree (Moringa oleifera) Leaf extract and phytase in Nile Tilapia (Oreochromis niloticus) highlighting the antioxidant, anti-inflammatory, and anti-apoptotic activities. Veterinary Research Communications, 49, 135. https://doi.org/10.1007/s11259-025-10672-5
  13. Himu, H.A. & Raihan, A. (2023). A review of the effects of intensive poultry production on the environment and human health. Journal of Veterinary Science and Animal Husbandry, 11(2), 203. https://www.annexpublishers.com/articles/JVSAH/11203-Intensive-Poultry-Production.pdf
  14. Jiménez-Aguilar, D.M. & Grusak, M.A. (2017). Minerals, vitamin C, phenolics, flavonoids and antioxidant activity of Amaranthus leafy vegetables. Journal of Food Composition and Analysis, 58, 33-39. https://doi.org/10.1016/j.jfca.2017.01.005
  15. Kashyap, P., Kumar, S., Riar, C.S., Jindal, N., Baniwal, P., Guiné, R.P., Correia, P.M., Mehra, R. and Kumar, H. (2022). Recent advances in Drumstick (Moringa oleifera) leaves bioactive compounds: Composition, health benefits, bioaccessibility, and dietary applications. Antioxidants, 11(2), 402. https://doi.org/10.3390/antiox11020402
  16. Khan, I.U., Shah, A.A., Sahibzada, F.A., Hayyat, A., Nazar, M., Mobashar, M., Tariq, A. & Sultana, N. (2019). Carcass characteristics and serum biochemical profile of Japanese quail by the supplementation of pine needles and vitamin E powder. Biologia, 74, 993-1000. https://doi.org/10.2478/s11756-019-00225-y
  17. Khan, R., Jolly, R., Fatima, T. & Shakir, M., (2022). Extraction processes for deriving cellulose: A comprehensive review on green approaches. Polymers for Advanced Technologies, 33(7), 2069-2090. https://doi.org/10.1002/pat.5678
  18. Menichetti, F., Berteotti, C., Schirinzi, V., Poli, C., Arrighi, R. & Leone, A. (2025). Moringa oleifera and Blood Pressure: Evidence and Potential Mechanisms. Nutrients, 17(7), 1258. https://doi.org/10.3390/nu17071258
  19. Maharjan, P., Martinez, D.A., Weil, J., Suesuttajit, N., Umberson, C., Mullenix, G., Hilton, K.M., Beitia, A. & Coon, C.N. (2021). Physiological growth trend of current meat broilers and dietary protein and energy management approaches for sustainable broiler production. Animal, 15(S1), 100284. https://doi.org/10.1016/j.animal.2021.100284
  20. Mahfuz, S. & Piao, X.S., (2019). Application of Moringa (Moringa oleifera) as natural feed supplement in poultry diets. Animals, 9(7), 431. https://doi.org/10.3390/ani9070431
  21. McGaw, L. (2025). Use of plant-derived extracts and bioactive compound mixtures against multidrug resistant bacteria affecting animal health and production. In Rai, M. (ed.). Fighting Multidrug Resistance with Herbal Extracts, Essential Oils and Their Components (pp. 291-311). Academic Press. https://doi.org/10.1016/B978-0-443-29044-2.00014-X
  22. Melesse, A., Masebo, M. & Abebe, A. (2018). The substitution effect of noug seed (Guizotia abyssinica) cake with cassava leaf (Manihot escutulata C.) meal on feed intake, growth performance, and carcass traits in broiler chickens. Journal of Animal Husbandry and Dairy Science, 2(2), 1-9. https://doi.org/10.22259/2637-5354.0202001
  23. Mesfin, Y.M., Mitiku, B.A. & Tamrat Admasu, H. (2024). Veterinary drug residues in food products of animal origin and their public health consequences: A review. Veterinary Medicine and Science, 10(6), e70049. https://doi.org/10.1002/vms3.70049
  24. Mohamed Kamil, M.A.I., Hanif Reduan, M.F., Mohd Noor, M.A., Abu-Bakar, L., Shamsuddin, S.H., Mohd Azmi, A.F., Mahamud, S.N.A., Hadi Roslan, M.R., Gilbert, B.S. & Abdul Hamid, F.F. (2024). The Effects of Piper Betle and Alpinia galanga Feed Additives on Growth Performance, Carcass Characteristics and Histomorphometry of Small Intestine in Broilers. Philippine Journal of Veterinary Medicine, 61(2). https://pjvm-ph.org/ana29052024/
  25. Nantapo, C.W.T., Muchenje, V., Marume, U. & Hoffman, L.C. (2024). Effect of phytogenic Moringa oleifera leaf powder on performance, carcass characteristics, immune indicators, gut microbial population and economic viability of broiler chickens. Discover Agriculture, 2, 85. https://doi.org/10.1007/s44279-024-00104-4
  26. Rossi, B., Toschi, A., Piva, A. & Grilli, E. (2020). Single components of botanicals and nature-identical compounds as a non-antibiotic strategy to ameliorate health status and improve performance in poultry and pigs. Nutrition Research Reviews, 33(2), 218-234. https://doi.org/10.1017/S0954422420000013
  27. Shah, A.A., Khan, I.U., Sahibzada, F.A., Tauseef, I., Kalsoom, U.E. & Sultana, N. (2019). Biological and biochemical characteristics of male reproductive system, serum metabolites and carcass quality of Japanese quails by the supplementation of Pinus ponderosa leaves and α‐tocopherol acetate. Reproduction in Domestic Animals, 54(10), 1348-1356. https://doi.org/10.1111/rda.13521
  28. Shah, A.A., Khan, M.S., Khan, S., Ahmad, N., Alhidary, I.A., Khan, R.U. & Shao, T., (2016). Effect of different levels of alpha tocopherol on performance traits, serum antioxidant enzymes, and trace elements in Japanese quail (Coturnix coturnix japonica) under low ambient temperature. Revista Brasileira de Zootecnia, 45(10), 622-626. https://doi.org/10.1590/S1806-92902016001000007
  29. Shah, A.A., Liu, Z., Qian, C., Wu, J., Sultana, N. & Zhong, X. (2020). Potential effect of the microbial fermented feed utilization on physicochemical traits, antioxidant enzyme and trace mineral analysis in rabbit meat. Journal of animal physiology and animal nutrition, 104(3), 767-775. https://doi.org/10.1111/jpn.13252
  30. Shah, A.A., Wu, J., Qian, C., Liu, Z., Mobashar, M., Tao, Z., Zhang, X. & Zhong, X. (2020). Ensiling of whole‐plant hybrid pennisetum with natamycin and Lactobacillus plantarum impacts on fermentation characteristics and meta‐genomic microbial community at low temperature. Journal of the Science of Food and Agriculture, 100(8), 3378-3385. https://doi.org/10.1002/jsfa.10371
  31. Singh, P., Bakshi, M. & Anmol, A. (2024). Natural plant extracts as a sustainable alternative to synthetic plant growth regulators: A review. International Journal of Advanced Biochemistry Research, 8(7), 281-287. https://doi.org/10.33545/26174693.2024.v8.i7d.1471
  32. Soni, K., Samtiya, M., Krishnan, V. & Dhewa, T. (2022). Antinutritional factors: Nutrient bioavailability and health beneficial effects. In: S. V., R., Praveen, S. (eds.). Conceptualizing Plant-Based Nutrition: Bioresources, Nutrients Repertoire and Bioavailability. Springer, Singapore. https://doi.org/10.1007/978-981-19-4590-8_8
  33. Zanu, H.K., Asiedu, P., Tampuori, M., Abada, M. & Asante, I. (2012). Possibilities of using Moringa (Moringa oleifera) leaf meal as a partial substitute for fishmeal in broiler chickens diets. Online Journal of Animal and Feed Research, 2(1), 70-75. https://www.ojafr.ir/main/attachments/article/82/OJAFR,%20B14,%2070-75,%202012.pdf

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