Skip to main content Skip to main navigation menu Skip to site footer
Review article
Austral J. Vet. Sci.
Vol 57, e5705 (2025)

Advancements and challenges in artificial insemination techniques for wild ruminant conservation: A review

1 Escuela de Medicina Veterinaria, Facultad de Medicina Veterinaria, Universidad San Sebastián, sede Concepción, Chile.
2 Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile.
3 Departamento de Fomento de la Producción Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, Chile.
Keywords: Biodiversity Ruminants Endangered Reproductive Biotechnologies Insemination

Submitted: 2024-09-09

Accepted: 2024-12-24

Published: 2025-04-10

*Corresponding author:
veterinariaconstanza@gmail.com

How to Cite

Rebolledo, P. C., Wong, Y. S. ., Veraguas-Dávila, D., Echeverry, D. M., Cartes, D. A., & Aguilera, C. J. (2025). Advancements and challenges in artificial insemination techniques for wild ruminant conservation: A review. Austral Journal of Veterinary Sciences, 57(1), e5705. https://doi.org/10.4206/ajvs.57.05

Abstract

Biodiversity is increasingly threatened by intensive agriculture, environmental pollution, climate change, and habitat loss, and many mammal species, including ruminants, have disappeared or are threatened with extinction. Therefore, reproductive biotechnologies represent an important alternative for the conservation of endangered species, being fixed-time artificial insemination (FTAI) the most widely used method, which has been extrapolated from livestock to wild ruminants. The main benefit of artificial insemination is the maintenance of the genetic diversity of populations through the preservation and use of semen from genetically valuable individuals. Variables, such as hormones and semen condition, can modify the efficiency of FTAI in domestic and wild animals. The aim of this review was to evaluate the different protocols and variations that have been reported in FTAI applied to different species of wild ruminants.

Downloads

Download data is not yet available.

References

  1. Abreu, C.O. (2006). Efeito da Vitamina E na Criopreservação de Sêmen de Veado-Bororó-do-Sul (Mazama nana). [Ph.D. Thesis, Universidade Estadual Paulista, Jaboticabal, Brasil].
  2. Abril-Sánchez, A., Crosignani, N., Freitas-de-Melo, A., Terrazas, A., Damián, J., Beracochea, F., Silveira, P. & Ungerfeld, R. (2018). Sedation or anaesthesia decrease the stress response to electroejaculation and improve the quality of the collected semen in goat bucks. Animal, 12 (12), 1598-2608. https://doi.org/10.1017/S1751731118000320
  3. Abril-Sánchez, S., Freitas-de-Melo, A., Giriboni, J., Santiago-Moreno, J. & Ungerfeld, R. (2019). Sperm collection by electroejaculation in small ruminants: A review on welfare problems and alternative techniques. Animal Reproduction Science, 205, 1-9. https://doi.org/10.1016/j.anireprosci.2019.03.023
  4. Acosta, G. (2001). Efecto de la fragmentación del bosque nativo en la conservación de Oncifelis guigna y Pseudalopex culpaeus en Chile central [Tesis de magíster, Universidad de Chile]. Repositorio Académico de la Universidad de Chile. https://repositorio.uchile.cl/handle/2250/106679
  5. Aguilera, M., & Silva, J. F. (1997). Especies y biodiversidad. Interciencia, 22(6), 299–306.
  6. Aleuy, O. (2008). Caracterización de medidas testiculares y semen de pudú (Pudu pudu) obtenido con un protocolo combinado de masaje digital transrectal y electroeyaculación durante su época reproductiva [Memoria de título, Universidad Austral de Chile, Facultad de Ciencias Veterinarias, Instituto de Reproducción Animal].
  7. Aller, J., Fernandez, O., & Sanchez, E. (2009). Fixed-time artificial insemination in red deer (Cervus elaphus) in Argentina. Animal reproduction science, 115(1–4), 312–316. https://doi.org/10.1016/j.anireprosci.2008.11.018
  8. Ambarcioglu, P., Mavridis, D., Yazlik, M., Vural, R., Akcil, M., Gurcan, S. Comparison of synchronisation protocols on pregnancy rate in dairy cows and heifers: A systematic review and network meta-analysis. Journal of Hellenic Veterinary Medical Society, 74 (2), 5657-5666. https://doi.org/10.12681/jhvms.29829
  9. Anzalone, D. A., Palazzese, L., Iuso, D., Martino, G., & Loi, P. (2018). Freeze-dried spermatozoa: An alternative biobanking option for endangered species. Animal reproduction science, 190, 85–93. https://doi.org/10.1016/j.anireprosci.2018.01.010
  10. Arif, A., Maulana, T., Kaiin, E., Purwantara, B., Arifiantini, R. & Memili, E. (2020). Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen. Veterinary World, 13 (11), 2422-2428. https://doi.org/10.14202/vetworld.2020.2422-2428
  11. Asher, G., Berg, D., & Evans, G. (2000). Storage of semen and artificial insemination in deer. Animal Reproduction Science, 62(1–3), 195–211. https://doi.org/10.1016/s0378-4320(00)00159-7
  12. Asher, G., Kraemer, D., Magyar, S., Brunner, M., Moerbe, R., & Giaquinto, M. (1990). Intrauterine insemination of farmed fallow deer (Dama dama) with frozen-thawed semen via laparoscopy. Theriogenology, 34(3), 569–577. https://doi.org/10.1016/0093-691x(90)90012-i
  13. Asher, G., Morrow, C., Jabbour, H., Mulley, R., Veldhuizen, F., & Langridge, M. (1992). Laparoscopic intra-uterine insemination of fallow deer with frozen-thawed or fresh semen after synchronisation with CIDR devices. New Zealand Veterinary Journal, 40(1), 8–14. https://doi.org/10.1080/00480169.1992.35689
  14. Asher, G. W., Peterson, A. J., & Duganzich, D. (1989). Adrenal and ovarian sources of progesterone secretion in young female fallow deer, Dama dama. Reproduction, 85(2), 667-675. https://doi.org/10.1530/jrf.0.0850667
  15. Assumpção, T. I., & Santos, A. L. Q. (2017). Cryopreservation of genetic material collected post-mortem from male gray brocket deer Mazama gouazoubira Fischer, 1814 [J]. International Journal of Current Science and Technology, 5(9), 510-512.
  16. Ballari, S., Pastore, H., & Varela, D. (2019). Pudu puda: Categorización de los mamíferos de Argentina. https://cma.sarem.org.ar/es/especie-nativa/pudu-puda
  17. Baruselli, P., Ferreira, G., Crepaldi, G., Chechin, B. & Santos, A. (2022). FTAI in numbers: evolution and future projection. Revista Brasileira de Reproducao Animal, 46 (2), 76-83.
  18. Becaluba, F. (2006). Métodos de sincronización de celos en bovinos. Sitio argentino de Producción Animal. Especialista en reproducción, Bs. Argentina.
  19. Black-Decima, P., Saucedo, C., Corti, P., Díaz, N., Fernandez, R., Geist, V., Gill, R., Gizejewski, Z., Jiménez, J., Pastore, H., & Wittmer, H. (2015). IUCN Red List of Threatened Species: Hippocamelus bisulcus. IUCN Red List of Threatened Species. https://www.iucnredlist.org/es
  20. Boever, J., Knox, D., Merilan, C., & Read, B. (1980). Estrus induction and artificial insemination with successful pregnancy in Speke’s gazelle. Proceedings of the 9th International Congress of Animal Reproduction and Artificial Insemination, Madrid, Spain, 2, 565–569.
  21. Borgelt, J., Dorber, M., Høiberg, M. A., & Verones, F. (2022). More than half of data deficient species predicted to be threatened by extinction. Communications Biology, 5(1), 679. https://doi.org/10.1038/s42003-022-03638-9
  22. Bott, N. I. (2018). Reproductive management of reindeer (Rangifer tarandus). American Association of Bovine Practitioners Conference Proceedings, 188–191. https://doi.org/10.21423/aabppro20183141
  23. Ceballos, G., Ehrlich, P. R., Barnosky, A. D., García, A., Pringle, R. M., & Palmer, T. M. (2015). Accelerated modern human–induced species losses: Entering the sixth mass extinction. Science advances, 1(5), e1400253. https://doi.org/10.1126/sciadv.1400253
  24. Celi, P. (2011). Oxidative stress in ruminants. In: Mandelker, L., Vajdovich, P. (eds) Studies on veterinary medicine. Oxidative Stress in Applied Basic Research and Clinical Practice. Humana Press, Totowa, NJ191–231. https://doi.org/10.1007/978-1-61779-071-3_13
  25. Christie, V. (2008). Manejo de inseminación artificial Intra-cervical con semen fresco en ovinos de la región de Magallanes [Tesis doctoral, Universidad de Magallanes, Facultad de Ciencias].
  26. Clemente-Sánchez, F., Gallegos-Sánchez, J., & Cortéz-Romero, C. (2017). Manual de reproducción asistida para el venado. Colegio de Postgraduados, Laboratorio de Reproducción Animal, Campus San Luis Potosí, Versión, 4, 18-52.
  27. Coloma, M., Toledano-Díaz, A., López-Sebastián, A., & Santiago-Moreno, J. (2010). The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod. Theriogenology, 73(7), 900–908. https://doi.org/10.1016/j.theriogenology.2009.11.014
  28. Comizzoli, P., Mermillod, P., Cognie, Y., Chai, N., Legendre, X., & Mauget, R. (2001). Successful in vitro production of embryos in the red deer (Cervus elaphus) and the sika deer (Cervus nippon). Theriogenology, 55(2), 649–659. https://doi.org/10.1016/s0093-691x(01)00433-2
  29. Corti, P., Wittmer, H. U., & Festa-Bianchet, M. (2010). Dynamics of a small population of endangered huemul deer (Hippocamelus bisulcus) in Chilean Patagonia. Journal of Mammalogy, 91(3), 690-697. https://doi.org/10.1644/09-mamm-a-047.1
  30. Cseh, S., & Solti, L. (2000). Importance of assisted reproductive technologies in the conservation of wild, rare or indigenous ungulates. Acta Veterinaria Hungarica, 48(3), 313–323. https://doi.org/10.1556/avet.48.2000.3.8
  31. Cseh, S., Faigl, V., & Amiridis, G. (2012). Semen processing and artificial insemination in health management of small ruminants. Animal reproduction science, 130(3–4), 187–192. https://doi.org/10.1016/j.anireprosci.2012.01.014
  32. del Monte-Luna, P., Lluch-Belda, D., & Arreguín-Sánchez, F. (2007). Examen de la conservación y el aprovechamiento de los recursos vivos. Interciencia, 32(1), 61–65.
  33. Densmore, M. A., Bowen, M. J., Magyar, S. J., Amoss Jr, M. S., Robinson, R. M., Harms, P. G., & Kraemer, D. C. (1987). Artificial insemination with frozen, thawed semen and pregnancy diagnosis in addax (Addax nasomaculatus). Zoo biology, 6(1), 21–29. https://doi.org/10.1002/zoo.1430060104
  34. Díaz Duque, N. A., & López Castaño, P. A. (2018). Protocolos de criopreservación de semen bovino [Tesis de licenciatura, Universidad Tecnológica de Pereira]. Repositorio Institucional de la Universidad Tecnológica de Pereira.
  35. Díaz, G., Galina, C., Basurto, C., & Ochoa, G. (2002). Efecto de la progesterona natural con o sin la adición de benzoato de estradiol sobre la presentación de celo, ovulación y gestación en animales tipo Bos indicus en el trópico mexicano. Archivos de medicina veterinaria, 34(2), 283–286. https://doi.org/10.4067/s0301-732x2002000200009
  36. Dobson, H., Walker, S., Morris, M., Routly, J., & Smith, R. (2008). Why is it getting more difficult to successfully artificially inseminate dairy cows? Animal, 2(8), 1104–1111. https://doi.org/10.1017/s175173110800236x
  37. Dott, H. M., & Utsi, M. N. P. (1973). Artificial insemination of Reindeer (Rangifer tarandus). Journal of Zoology, 170(4), 505-508. https://doi.org/10.1111/j.1469-7998.1973.tb05065.x
  38. Duarte, G. S., Galindo, D. J., Baldini, M. H. M., da Fonseca, J. F., Duarte, J. M. B., & Oliveira, M. E. F. (2023). Transcervical artificial insemination in the brown brocket deer (Subulo gouazoubira): a promising method for assisted reproduction in deer. Scientific Reports, 13(1), 17369. https://doi.org/10.1038/s41598-023-43392-4
  39. Duarte, J. M. B., Merino, M. L., Gonzalez, S., Nunes, A. L. V., Garcia, J. M., Szabó, M. P. J., Pandolfi, J. R., Arantes, I. G., Antonio Do Nascimento, A., Machado, R. Z., Arauco, J. P., Catão-Díaz, J. L., Werther, K., García, J. E., da Silva, R. J., & Matushima, E. R. (2001). Order artiodactyla, family Cervidae (deer). In M.E. Fowler (Ed.) Biology, Medicine, and Surgery of South American Wild Animals (pp. 402-422). https://doi.org/10.1002/9780470376980.ch35
  40. Duarte, J. M. B., & Garcia, J. M. (1995). Assistant reproduction in Brazilian cervidae. Revista Brasileira de Reproducao Animal (Brazil), 19(1).
  41. Engdawork, A., Belayhun, T., & Aseged, T. (2024). The Role of Reproductive Technologies and Cryopreservation of Genetic Materials in the Conservation of Animal Genetic Resources, A Review. Ecological Genetics and Genomics, 100250. https://doi.org/10.1016/j.egg.2024.100250
  42. Eski, F., Kurt, S. & Ayvazoglu, P. (2021). Effect of different estrus synchronization protocols on estrus and pregnancy rates, oxidative stress and some biochemical parameters in Hair goats. Small Ruminant Research, 106348. https://doi.org/10.1016/j.smallrumres.2021.106348
  43. Flueck, W. T., Smith-Flueck, J. A. M., Escobar, M. E., Zuliani, M. E., Fuchs, B., Heffelfinger, J. R., Black-Decima, P., Gizejewski, Z., Vidal, F., Barrio, J., Molinuevo, S. M., Monjeau, A. J., Hoby, S., & Jiménez, J. E. (2023). Review of Historical and Zooarchaeological Data to Trace Past Biogeographic Distribution of Endangered Huemul (Hippocamelus bisulcus) to Enhance Conservation Strategies. Conservation, 3(4), 569-594. https://doi.org/10.3390/conservation3040036
  44. Flueck, W. T., Smith-Flueck, J. A. M., Escobar, M. E., Zuliani, M., Fuchs, B., Geist, V., Heffelfinger, J. R., Black-Decima, P., Gizejewski, Z., & Vidal, F. (2022). Loss of migratory traditions makes the endangered Patagonian huemul deer a year-round refugee in its summer habitat. Conservation, 2(2), 322–348. https://doi.org/10.3390/conservation2020023
  45. Folch, J., Cocero, M. J., Chesné, P., Alabart, J. L., Domínguez, V., Cognié, Y., Roche, A., Fernández-Arias, A., Martí, J., Sánchez, P., Echegoyen, E., Beckers, J. F., Sánchez Bonastre, A., & Vignon, X. (2009). First birth of an animal from an extinct subspecies (Capra pyrenaica pyrenaica) by cloning. Theriogenology, 71(6), 1026–1034. https://doi.org/10.1016/j.theriogenology.2008.11.005
  46. Fonseca, J. F., Oliveira, M. E. F., Brandão, F. Z., Batista, R. I. T. P., Garcia, A. R., Bartlewski, P. M., & Souza-Fabjan, J. M. G. (2019). Non-surgical embryo transfer in goats and sheep: The Brazilian experience. Reproduction, Fertility and Development, 31(1), 17–26. https://doi.org/10.1071/RD18324
  47. Frid, A. (1994). Observations on habitat use and social organization of a huemul Hippocamelus bisulcus coastal population in Chile. Biological Conservation, 67(1), 13–19. https://doi.org/10.1016/0006-3207(94)90003-5
  48. Fumagalli, F., Villagrán, M., Damián, J. P., & Ungerfeld, R. (2012). Physiological and biochemical parameters in response to electroejaculation in adult and yearling anesthetized pampas deer (Ozotoceros bezoarticus) males. Reproduction in domestic animals, 47(2), 308-312. https://doi.org/10.1111/j.1439-0531.2011.01859.x
  49. Gibbons, A. E., Fernandez, J., Bruno-Galarraga, M. M., Spinelli, M. V., & Cueto, M. I. (2019). Technical recommendations for artificial insemination in sheep. Animal reproduction, 16(4), 803–809. https://doi.org/10.21451/1984-3143-ar2018-0129
  50. Gibbons, A., Cueto, M. (1995). Manual de inseminación artificial en la especie ovina. INTA Bariloche.
  51. Gillan, L., Maxwell, W. C., & Evans, G. (2004). Preservation and evaluation of semen for artificial insemination. Reproduction, Fertility and Development, 16(4), 447–454. https://doi.org/10.1071/rd04034
  52. Gillis, J., Holt, W., Yon, L., Myers, G., Schnellbacher, R., Holly, R., Love, D. & Penfold, L. (2023). Effects of exogenous oxytocin on the semen characteristics of the Indonesian wild cattle, banteng (Bos javanicus), collected by electroejaculation: Implications for semen collection techniques and genome resource banking. Theriogenology Wild, 3, 100044. https://doi.org/10.1016/j.therwi.2023.100044
  53. Gomendio, M., Roldán, E., Garde, J., & Espeso, G. (2006). El papel de las biotecnologías reproductivas en la conservación animal. Ecosistemas, 15(2).
  54. Gonzales, H., Scotto, C., Davalos, R., Gonzales, H., & Scotto, C. (2019). Biotecnología reproductiva en animales silvestres. Reproductive Biotechnology in Wild Animals, 9, 69–82.
  55. Gonzalez, S. Álvarez-Valin, F., & Maldonado, J. E. (2002). Morphometric differentiation of endangered pampas deer (Ozotoceros bezoarticus), with description of new subspecies from Uruguay. Journal of Mammalogy, 83, 1127–1140. https://doi.org/10.1644/1545-1542(2002)083<1127:mdoepd>2.0.co;2
  56. González, S., Maldonado, J. E., Leonard, J. A., Vilà, C., Duarte, J. M. B., Merino, M., Brum-Zorrilla, N., & Wayne, R. K. (1998). Conservation genetics of the endangered Pampas deer (Ozotoceros bezoarticus). Molecular Ecology, 7(1), 47–56. ttps://doi.org/10.1046/j.1365-294x.1998.00303.x
  57. Goss, R. J. (1983). Deer antlers: regeneration, function and evolution. Academic Press Inc. New York.
  58. Gottschall, C. S., de Almeida, M. R., Tolotti, F., Magero, J., Bittencourt, H. R., Mattos, R. C., & Gregory, R. M. (2012). Avaliação do desempenho reprodutivo de vacas de corte lactantes submetidas à IATF a partir da aplicação do GnRH, da manifestação estral, da reutilização de dispositivos intravaginais e da condição corporal. Acta Scientiae Veterinariae, 40(1), 1–10.
  59. Haigh, J., & Bowen, G. (1991). Artificial insemination of red deer (Cervus elaphus) with frozen–thawed wapiti semen. Reproduction, 93(1), 119–123. https://doi.org/10.1530/jrf.0.0930119
  60. Hameed, N., Irfan-ur-Rehman, M., Zubair, M. & Hassan, S. (2021). Approaches of estrous synchronization in sheep: developments during the last two decades: a review. Tropical Animal Health and Production, 53(5), 485. https://doi.org/10.1007/s11250-021-02932-8
  61. Hernández-Coronado, C., Rosales-Torres, A., Vázquez-López, S. & Guzmán-Sánchez, A. (2023). Synchronization of estrus and ovulation in bovine females. Endocrine bases and treatments used. Abanico Veterinario, 13 (1), 1-37. https://doi.org/10.21929/abavet2023.16
  62. Hewett, A. M., Stoffel, M. A., Peters, L., Johnston, S. E., & Pemberton, J. M. (2023). Selection, recombination and population history effects on runs of homozygosity (ROH) in wild red deer (Cervus elaphus). Heredity, 130(4), 242-250. https://doi.org/10.1038/s41437-023-00602-z
  63. Hidalgo-Hermoso, E., Verasay Caviedes, S., Pizarro-Lucero, J., Cabello, J., Vicencio, R., Celis, S., Ortiz, C., Kemec, I., Abuhadba-Mediano, N., Asencio, R., & others. (2024). High Exposure to Livestock Pathogens in Southern Pudu (Pudu puda) from Chile. Animals, 14(4), 526. https://doi.org/10.3390/ani14040526
  64. Hidalgo, M. M. T., Marques de Almeida, A. B., Zito de Moraes, F. L., Marubayashi, R. Y. P., Barreiros, T. R. R., & Martins, M. I. M. (2022). Influence of Sperm Post-Thaw Subpopulations of Angus and Nelore Bulls on Pregnancy Rates by Fixed-Time Artificial Insemination. CryoLetters, 43(3), 140–149. https://doi.org/10.54680/fr22310110312
  65. Holt, W., Abaigar, T., & Jabbour, H. (1996). Oestrous synchronization, semen preservation and artificial insemination in the Mohor gazelle (Gazella dama mhorr) for the establishment of a genome resource bank programme. Reproduction, fertility and development, 8(8), 1215–1222. https://doi.org/10.1071/rd9961215
  66. Holt, W., Moore, H., North, R., Hartman, T., & Hodges, J. (1988). Hormonal and behavioural detection of oestrus in blackbuck, Antilope cervicapra, and successful artificial insemination with fresh and frozen semen. Reproduction, 82(2), 717–725. https://doi.org/10.1530/jrf.0.0820717
  67. Humble, E., Stoffel, M. A., Dicks, K., Ball, A. D., Gooley, R. M., Chuven, J., Pusey, R., Remeithi, M. A., Koepfli, K.-P., Pukazhenthi, B., Senn, H., & Ogden, R. (2023). Conservation management strategy impacts inbreeding and mutation load in scimitar-horned oryx. Proceedings of the National Academy of Sciences, 120(18), e2210756120. https://doi.org/10.1073/pnas.2210756120
  68. Johnston, S., Blyde, D., Pedrana, R., & Gibbs, A. (2000). Laparoscopic intrauterine insemination in Barbary sheep (Ammotragus lervia). Australian Veterinary Journal, 78(10), 714–717. https://doi.org/10.1111/j.1751-0813.2000.tb10414.x
  69. Jorquera-Jaramillo, C., Vega, J. A., Aburto, J., Martinez-Tilleria, K., Leon, M. F., Perez, M. A., Gaymer, C. F., & Squeo, F. A. (2012). Biodiversity conservation in Chile: New challenges and opportunities in terrestrial and marine coastal ecosystems. Revista Chilena de Historia Natural, 85(3), 267–280. https://doi.org/10.4067/s0716-078x2012000300002
  70. Korzekwa, A. J., & Kotlarczyk, A. M. (2021). Artificial reproductive technology (ART) applied to female cervids adapted from domestic ruminants. Animals, 11(10), 2933. https://doi.org/10.3390/ani11102933
  71. Krepschi, V., Polegato, B., Zanetti, E. & Duarte, J. (2013). Fecal progestins during pregnancy and postpartum periods of captive red brocket deer (Mazama americana). Animal Reproduction Science. 137, 62–68. https://doi.org/10.1016/j.anireprosci.2012.11.016
  72. Ladd, R., Crouthers, R., Brook, S., & Eames, J. C. (2022). Reviewing the status and demise of the Endangered Eld’s deer and identifying priority sites and conservation actions in Cambodia. Mammalia, 86(5), 407–421. https://doi.org/10.1515/mammalia-2021-0151
  73. Lindeberg, H., Nikitkina, E., Nagy, S., Musidray, A., Shiryaev, G., Kumpula, J., & Holand, Ø. (2021). Potential applications of assisted reproductive technologies (ART) in reindeer (Rangifer tarandus). Animal Reproduction Science, 235, 106890. https://doi.org/10.1016/j.anireprosci.2021.106890
  74. Longobardi, V., Kosior, M., Pagano, N., Fatone, G., Starpoli, A., Vassetti, A., Vinale, F., Campanile, G. & Gasparrini, B. (2020). Changes in Bull Semen Metabolome in Relation to Cryopreservation and Fertility. Animals, 10 (6), 1065. https://doi.org/10.3390/ani10061065
  75. Lucy, M. C. (2019). Stress, strain, and pregnancy outcome in postpartum cows. Animal Reproduction, 16, 455–464. https://doi.org/10.21451/1984-3143-ar2019-0063
  76. Lv, C., Wu, G., Hong, Q., & Quan, G. (2019). Spermatozoa cryopreservation: State of art and future in small ruminants. Biopreservation and biobanking, 17(2), 171–182. https://doi.org/10.1089/bio.2018.0113
  77. Magyar, S. J., Biediger, T., Hodges, C., Kraemer, D. C., & Seager, S. W. J. (1989). A method of artificial insemination in captive White-tailed deer (Odocoileus virginianus). Theriogenology, 31(5), 1075-1079. https://doi.org/10.1016/0093-691x(89)90491-3
  78. Malik, A., Fazil, M., Khatun, A., Athar, H., Khan, H. & Shah, R. (2023). Comparative evaluation of natural tupping with fixed-time laparoscopic and cervical insemination techniques using chilled semen in estrous syncrhronized sheep. Indian Journal of Animal Sciences, 93 (12), 1160-1165. https://doi.org/10.56093/ijans.v93i12.109893
  79. Martínez-Barbitta, M., Gil, J., Costa, G., Pombo, I., Peñagaricano, J., Lutz, M., Freire, A., & Cavestany, D. (2015). Evaluación de dos formulaciones de progesterona en protocolos HeatSynch en vacas Holando lactando en sistemas pastoriles. Veterinaria (Montevideo), 51(197-200), 108-115.
  80. Mastromonaco, G. & Songsasen, N. (2020). Reproductive technologies for the conservation of wildlife and endangered species. In: Presicce, G. (ed) Reproductive Technologies in Animals (pp. 99-117). Academic Press.
  81. Matsuda, D., Bellem, A., Gartley, C., Madison, V., King, W., Liptrap, R. y Goodrowe. (1996). Endocrine and behavioral events of estrous cyclicity and synchronization in wood bison (Bison bison athabascae). Theriogenology, 45(8), 1429-1441. https://doi.org/10.1016/0093-691x(96)85667-6
  82. McDougall, P. T., Réale, D., Sol, D., & Reader, S. M. (2006). Wildlife conservation and animal temperament: causes and consequences of evolutionary change for captive, reintroduced, and wild populations. Animal Conservation, 9(1), 39-48. https://doi.org/10.1111/j.1469-1795.2005.00004.x
  83. Menchaca, A., & Rubianes, E. (2004). New treatments associated with timed artificial insemination in small ruminants. Reproduction, Fertility and Development, 16(4), 403–413. https://doi.org/10.1071/RD04037
  84. Ming, Y., Zainuddin, Z. Z., & others. (2000). Evaluation of semen collected by electroejaculation from captive lesser Malay chevrotain (Tragulus javanicus). Journal of Zoo and Wildlife Medicine, 31(2), 164–167. https://doi.org/10.1638/1042-7260(2000)031[0164:eoscbe]2.0.co;2
  85. Morrow, C., Penfold, L., & Wolfe, B. (2009). Artificial insemination in deer and non-domestic bovids. Theriogenology, 71(1), 149–165. https://doi.org/10.1016/j.theriogenology.2008.09.001
  86. Morrow, C., Wolfe, B., Roth, T. L., Wildt, D. E., Bush, M., Blumer, E., Atkinson, M., & Monfort, S. L. (2000). Comparing ovulation synchronization protocols for artificial insemination in the scimitar-horned oryx (Oryx dammah). Animal reproduction science, 59(1–2), 71–86. https://doi.org/10.1016/s0378-4320(00)00067-1
  87. Muñoz, S. (2008). Congelación de semen de pudú (Pudu pudu): Efecto de los diluyentes Tris y leche SB sobre semen diluido [Memoria de título, Universidad Austral de Chile].
  88. Mulley, R. C., Moore, N. W., & English, A. W. (1988). Successful uterine insemination of fallow deer with fresh and frozen semen. Theriogenology, 29(5), 1149-1153. https://doi.org/10.1016/s0093-691x(88)80040-2
  89. Mylrea, G. E., English, A. W., Mulley, R. C., & Evans, G. (1992). Artificial insemination of farmed chital deer. In Brown, R.D. (eds) The biology of deer (pp. 334–337). Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2782-3_70
  90. Northrup, J. M., Shafer, A. B., Anderson Jr, C. R., Coltman, D. W., & Wittemyer, G. (2014). Fine‐scale genetic correlates to condition and migration in a wild cervid. Evolutionary Applications, 7(8), 937-948. https://doi.org/10.1111/eva.12189
  91. Núñez, I., González-Gaudiano, É., & Barahona, A. (2003). La biodiversidad: Historia y contexto de un concepto. Interciencia, 28(7), 387–393.
  92. Oliveira, L., Valenza, A., Rodrigues, R., Anastacio, M., Barreiro, T., Lemos, J., Nora, J., Madureira, G., Henryli, A. & Sartori, R. (2021). Progesterone release profile and folicular development in Holstein cows receiving intravaginal progesterona devices. Theriogenology, 172 (15), 207-215. https://doi.org/10.1016/j.theriogenology.2021.07.001
  93. Pereira, R. J. G., Duarte, J. M. B., & Negrão, J. A. (2005). Seasonal changes in fecal testosterone concentrations and their relationship to the reproductive behavior, antler cycle and grouping patterns in free-ranging male Pampas deer (Ozotoceros bezoarticus bezoarticus). Theriogenology, 63(8), 2113-2125. https://doi.org/10.1016/j.theriogenology.2004.08.014
  94. Pérez-Ruiz, E., Quezada-Casasola, A., Carrera-Chávez, J., Álvarz-Holguin, A., Ochoa-Rivero, J., Chávez-Ruiz, M. & Román-Ponce, S. Función ovárica y respuesta a la sincronización del estro en ganado Criollo en Mexico. Revistas Mexicana de Ciencias Pecuarias, 13(2), 422-451. https://doi.org/10.22319/rmcp.v13i2.6032
  95. Pievani, T. (2014). The sixth mass extinction: Anthropocene and the human impact on biodiversity. Rendiconti Lincei, 25, 85–93. https://doi.org/10.1007/s12210-013-0258-9
  96. Pintus, E., & Ros-Santaella, J. (2014). Assisted reproductive technologies in deer (Artiodactyla, Cervidae): A review. Scientia agriculturae bohemica, 45(2), 136–146. ttps://doi.org/10.7160/sab.2014.450210
  97. Polegato, B. F., Zanetti, E. D. S., & Duarte, J. M. B. (2018). Monitoring ovarian cycles, pregnancy and post-partum in captive marsh deer (Blastocerus dichotomus) by measuring fecal steroids. Conservation Physiology, 6(2), cox073. https://doi.org/10.1093/conphys/cox073
  98. Redden, R., Neville, T., Black, D., Crosswhite, M., & Dahlen, C. (2023). Effect of progesterone infused controlled internal drug releasing (CIDR) device and timing of gonadotropin stimulation using P.G. 600 on reproductive success in ewes bred out of season. Translational Animal Science, 7 (1). https://doi.org/10.1093/tas/txad081
  99. Ribeiro-Peres, A., Munita-Barbosa, L., Yumi-Kanazawa, M., Mello-Martins, M., & Ferreira de Souza, F. (2014). Criopreservación de espermatozoides bovinos extraídos de la cola del epidídimo utilizando los métodos convencional y automatizado. Archivos de medicina veterinaria, 46(1), 31–38. https://doi.org/10.4067/s0301-732x2014000100005
  100. Rocha, M. S., Maia, A. L. R., Rangel, P. S. C., Oliveira, M. E. F., Fonseca, J. F., Oliveira, C. A., & Souza-Fabjan, J. M. (2022). Occurrence of premature regression of corpus luteum in MOET programs in Dorper ewes under subtropical climate. Livestock Science, 255, 104808. https://doi.org/10.1016/j.livsci.2021.104808
  101. Rodrigues, J., Ungerfeld, R., Alvarez, M., Goncalves, J., Oliveira, I., Lopes, V., Vieira, C., Pinto, P., Cunha, A., Ferreira, J., Zandonadi, F. (2020). Transcervical vs. laparotomy embryo collection in ewes: The effectiveness and welfare implications of each technique. Theriogenology, 153, 112-121. https://doi.org/10.1016/j.theriogenology.2020.05.004
  102. Rodríguez Sousa, A. A. (2018). Ciencia y divulgación sobre la sexta extinción masiva de biodiversidad, ¿es realmente el cambio climático el principal responsable? In R. Fernández-Reyes, D., & Rodrigo-Cano (Eds.) La comunicación de la mitigación y la adaptación al Cambio Climático (pp. 177-204). Sevilla: Egregius
  103. Rojas, M., Castro, R., Venegas, F., Álvarez, R., & Guillomot, M. (2016). Impacto de la biotecnología reproductiva en la conservación de los animales en riesgo de extinción. TecnoVet, 12(3), 9-15.
  104. Rola, L. D.; Zanetti, E. S.; Duarte, J. M. B. (2012). Avaliação de dois métodos para condicionamento e coleta de sêmen em quatro espécies do gênero Mazama. Pesquisa Veterinária Brasileira, 32, 658–662. https://doi.org/10.1590/s0100-736x2012000700013
  105. Rola, L. D., Buzanskas, M. E., Melo, L. M., Chaves, M. S., Freitas, V. J. F., & Duarte, J. M. B. (2021). Assisted reproductive technology in neotropical deer: A model approach to preserving genetic diversity. Animals, 11(7), 1961. https://doi.org/10.3390/ani11071961
  106. Rolf, H. J., & Fischer, K. (1990). Serum testosterone (T) and 5-α-dihydrotestosterone (DHT) in male fallow deer (Dama dama L.): seasonality and age dependence. Comparative Biochemistry and Physiology Part A: Physiology, 95(3), 445-452. https://doi.org/10.1016/0300-9629(90)90247-p
  107. Salamon, S., & Maxwell, W. M. C. (2000). Storage of ram semen. Animal reproduction science, 62(1–3), 77–111. https://doi.org/10.1016/s0378-4320(00)00155-x
  108. Sales Zlatar, F. A. & others. (2000). Inseminación artificial ovina en la XII Región: II parte. Punta Arenas, Chile: Boletín INIA - Instituto de Investigaciones Agropecuarias. no. 50.
  109. Santiago-Moreno, J. S., Díaz, A. T., Brunet, A. G., Pastor, A. P., & Sebastián, A. L. (2008). Synchronization of ovulation and artificial insemination protocol for Spanish ibex (Capra pyrenaica) based in progesterone and cloprostenol. Spanish journal of agricultural research, (1), 39–41. https://doi.org/10.5424/sjar/2008061-302
  110. Santiago-Moreno, J., Toledano-Díaz, A., Castaño, C., Velázquez, R., Bóveda, P., O'Brien, E., Peris-Frau, P., Pequeño, B., Martínez-Madrid, B., & Esteso, M. C. (2023). Sperm cryopreservation in wild small ruminants: morphometric, endocrine and molecular basis of cryoresistance. Animal, 17, 100741. https://doi.org/10.1016/j.animal.2023.100741
  111. Santiago-Moreno, J., Toledano-Díaz, A., Pulido-Pastor, A., Gómez-Brunet, A., & López-Sebastián, A. (2006). Birth of live Spanish ibex (Capra pyrenaica hispanica) derived from artificial insemination with epididymal spermatozoa retrieved after death. Theriogenology, 66(2), 283–291. https://doi.org/10.1016/j.theriogenology.2005.11.012
  112. Santiago-Moreno, J., Toledano-Díaz, A., Sookhthezary, A., Gómez-Guillamón, F., Salas, R., Pulido-Pastor, A. & Lopez-Sebastián, A. (2011). Effects of anesthetic protocols on electroejaculation variables of Iberian ibex (Capra pyrenaica). Research in Veterinary Science, 90 (1), 150-155. https://doi.org/10.1016/j.rvsc.2010.05.011
  113. Santos, E., Furlan, B. & Barbanti, J. (2010). Comparison of two methods of synchronization of estrus in brown brocket deer (Mazama gouazoubira). Animal Reproduction Science, 117 (3), 266-274. https://doi.org/10.1016/j.anireprosci.2009.05.010
  114. Santos, J. P., Acevedo, P., Carvalho, J., Queiros, J., Villamuelas, M., Fonseca, C., Gortazar, C., López-Olvera, J. R., & Vicente, J. (2018). The importance of intrinsic traits, environment and human activities in modulating stress levels in a wild ungulate. Ecological indicators, 89, 706–715. https://doi.org/10.1016/j.ecolind.2018.02.047
  115. Sathe, S. R. (2018). Laparoscopic artificial insemination technique in small ruminants—A procedure review. Frontiers in veterinary science, 5, 266. https://doi.org/10.3389/fvets.2018.00266
  116. Sempéré, A. J., Mauget, R., & Chemineau, P. (1992). Experimental induction of luteal cyclicity in roe deer (Capreolus capreolus). Reproduction, 96(1), 379-384. https://doi.org/10.1530/jrf.0.0960379
  117. Setiadi, D. R., Agil, M., Mulia, B. H., Widianti, A., Sajuthi, D., Manansang, J., Hastuti, Y. T., Liwa, S. R., & Arifiantini, R. (2023). Determination of artificial insemination timing in Banteng based on follicle size and uterine enlargement. Asian Journal of Conservation Biology, 12(1). https://doi.org/10.53562/ajcb.76733
  118. Silva-Rodríguez, E., Jiménez, J., & Pastore, H. (2016). IUCN Red List of Threatened Species: Pudu puda. IUCN Red List of Threatened Species. https://www.iucnredlist.org/es
  119. Souza-Fabjan, J., Oliveira, M., Guimarães, M., Brandão, F., Bartlewski, P., & Fonseca, J. (2023). Non-surgical artificial insemination and embryo recovery as safe tools for genetic preservation in small ruminants. Animal, 17, 100787. https://doi.org/10.1016/j.animal.2023.100787
  120. Suttie, J. M., Lincoln, G. A., & Kay, R. N. B. (1984). Endocrine control of antler growth in red deer stags. Reproduction, 71(1), 7-15. https://doi.org/10.1530/jrf.0.0710007
  121. Schams, D., & Barth, D. (1982). Annual profiles of reproductive hormones in peripheral plasma of the male roe deer (Capreolus capreolus). Reproduction, 66(2), 463-468. https://doi.org/10.1530/jrf.0.0660463
  122. Spanner, E., Graaf, S. & Rickard, J. (2024). Factors affecting the success of laparoscopic artificial insemination in sheep. Animal Reproduction Science, 264 107453. https://doi.org/10.1016/j.anireprosci.2024.107453
  123. Cursino, M. S. (2014). Análise comparativa de características seminais de Mazama cinzas brasileiros: Morfometria espermática e proteómica do plasma seminal [Tesis doctoral, Universidade Estadual Paulista (Unesp)].
  124. Sylla, L., Pistolesi, A., Corsi, I., Crociati, M., Stradaioli, G., & Monaci, M. (2021). Laparotomic intrauterine insemination with frozen-thawed semen in Sopravissana breed ewes in Central Italy. Italian journal of animal science, 20(1), 928–934. https://doi.org/10.1080/1828051x.2021.1918585
  125. Tanaka, Y., Americano, A., Galindo, D. & Duarte, J. (2020). Low invasive estrous synchronization protocol for wild animals: An example with melengestrol acetate in brown brocket deer (Mazama gouazoubira). Animal Reproduction, 17, 1–11. https://doi.org/10.1590/1984-3143-ar2020-0526
  126. Tanaka, Y., Herédias-Ribas, C. M., Baldini, M. H. M., Guevara, J. E. H., & Duarte, J. M. B. (2021). Evaluation of potential reproductive seasonality in brown brocket deer (Mazama gouazoubira) bucks. Theriogenology, 171, 104-112. https://doi.org/10.1016/j.theriogenology.2021.05.018
  127. Tellería, J. L. (2012). Introducción a la conservación de las especies. Tundra Ediciones.
  128. Timmins, R., Duckworth, J., Hedges, S., Steinmetz, R., & Pattanavibool, A. (2019). Bos javanicus. The IUCN Red List of Threatened Species 2008: e.T2888A9490684.
  129. Ungerfeld, R., Casuriaga, D., Giriboni, J., Freitas-de-Melo, A., Silveira, P., & Brandão, F. Z. (2018). Administration of cloprostenol and oxytocin before electroejaculation in goat bucks reduces the needed amount of electrical stimulation without affecting seminal quality. Theriogenology, 107, 1-5. https://doi.org/10.1016/j.theriogenology.2017.10.034
  130. Ungerfeld, R., López-Sebastián, A., Esteso, M., Pradiee, J., Toledano-Díaz, A., Castaño, C., Labrador, B., & Santiago-Moreno, J. (2015). Respuestas fisiológicas y características de los espermatozoides recogidos tras electroeyaculación o masaje ecoguiado transrectal de las glándulas sexuales accesorias en muflones (Ovis musimon) y cabras montesas (Capra pyrenaica) anestesiados. Theriogenology, 84(7), 1067–1074. https://doi.org/10.1016/j.theriogenology.2015.06.009
  131. Velasco Fuenmayor, J., & Ortega Soto, L. (2008). La inseminación artificial y su efecto sobre los índices de productividad parcial en fincas ganaderas de doble propósito. Revista Científica, 18(3), 278-283.
  132. Velasco, J. A. G., Sotomayor, L. M., Gómez, M. B., Masip, M. A., Reus, R., & Gutiérrez, S. A. (2022, abril 12). Congelación de esperma: Indicaciones, proceso, resultado y precio. Reproducción Asistida ORG. https://www.reproduccionasistida.org/congelacion-de-semen/
  133. Vidal, F., Smith-Flueck, J. A. M., Flueck, W. T., & Bartoš, L. (2012). Variation in reproduction of a temperate deer, the southern pudu (Pudu puda). Animal production science, 52(8), 735-740. https://doi.org/10.1071/an11364
  134. West, N. O., & Nordan, H. C. (1976). Hormonal regulation of reproduction and the antler cycle in the male Columbian black-tailed deer (Odocoileus hemionus columbianus). Part II. The effects of methallibure and hormone treatment. Canadian journal of zoology, 54(10), 1637-1656. https://doi.org/10.1139/z76-190
  135. Weber, M., & Gonzalez, S. (2003). Latin American deer diversity and conservation: a review of status and distribution. Ecoscience, 10(4), 443-454. https://doi.org/10.1080/11956860.2003.11682792
  136. Wiebke, M., Pieper, L., Gurler, H., Janowitz, U., Jung, M. & Schulze, M. (2023). Effect of using liquid semen on fertility in German Holstein Friesian dairy cattle: A randomized controlled clinical trial. Theriogenology, 199, 50-56. https://doi.org/10.1016/j.theriogenology.2023.01.012
  137. Wolfenson, L. I., Pereira, J. A., Ruzzante, D. E., Solé-Cava, A. M., McCracken, G. R., Gómez-Fernández, M. J., Pereyra, M. D., & Mirol, P. M. (2024). Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples. Scientific Reports, 14(1), 16169. https://doi.org/10.1038/s41598-024-67062-1
  138. WWF. (2022). ¿Qué es la sexta extinción masiva y qué podemos hacer al respecto? | Historias | Descubre WWF. https://www.worldwildlife.org/descubre-wwf/historias/que-es-la-sexta-extincion-masiva-y-que-podemos-hacer-al-respecto
  139. Yizengaw, L. (2017). Review on Estrus Synchronization and Its Application in Cattle. International Journal of Advanced Research in Biological Sciences, 4(4), 67-76. https://doi.org/10.22192/ijarbs.2017.04.04.010
  140. Zbyryt, A., Bubnicki, J. W., Kuijper, D. P., Dehnhard, M., Churski, M., & Schmidt, K. (2018). Do wild ungulates experience higher stress with humans than with large carnivores? Behavioral Ecology, 29(1), 19–30. https://doi.org/10.1093/beheco/arx142
  141. Zerbe, P., Clauss, M., Codron, D., Lackey, L., Rensch, E., Streich, J., Hatt, J. & Muller, D. (2012). Reproductive seasonality in captive wild ruminants: implications for biogeographical adaptation, photoperiodic control, and life history. Biological Reviews, 87 (4), 965-990. https://doi.org/10.1111/j.1469-185x.2012.00238.x

Make a Submission

Journal Metrics (2023) & Ranking

Impact Factor
0.5 (2024)
5 years Impact Factor
0.8
JCR Quartile
Q4
JIF Rank
134/170 (Veterinary Sciences)
SJR (2024)
0.244
SNIP (2024)
0.35

 


 

SCImago Journal & Country Rank

Indexed in




Publisher

Keywords