Skip to main content Skip to main navigation menu Skip to site footer
Short communication
Austral J. Vet. Sci.
Vol 56, 121-127 (2024)
DOI: 10.4206/ajvs.563.04

Molecular detection of haemobacteria in Colombian wild birds

1 Escuela de Medicina Veterinaria, Facultad de Ciencias Agrarias, Universidad de Antioquia, Medellín, Colombia.
2 Grupo de Investigación IMPRONTA, Universidad Cooperativa de Colombia, Colombia; Department of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, Virginia, US.
3 Research Group of Infectious Diseases, Zoonoses and Environment TestMol© Laboratory (GIZMOL), TESTMOL© SAS – Specialised Diagnostic Centre, Medellín, Colombia.
4 CENTAURO Research Group, Escuela de Medicina Veterinaria, Facultad de Ciencias Agrarias, Universidad de Antioquia, Medellín, Colombia.
Keywords: blood Exiguobacterium spp. E. coli hemobacteria Stenotrophomonas spp. wild bird

Submitted: 2024-07-15

Accepted: 2024-09-11

Published: 2024-11-22

*Corresponding author:
mariadelp.correa@udea.edu.co

How to Cite

Arroyave Pérez, A. O. ., Cifuentes Rincón, A. ., Jaramillo Delgado, I. L., & Correa Valencia, N. M. del P. (2024). Molecular detection of haemobacteria in Colombian wild birds. Austral Journal of Veterinary Sciences, 56(3), 121–127. https://doi.org/10.4206/ajvs.563.04

License

Copyright (c) 2024 Arley Onasis Arroyave Pérez, Analorena Cifuentes Rincón, Ingrid Lorena Jaramillo Delgado, Nathalia María del Pilar Correa Valencia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Colombia shows a high density and variety of bird species, making it one of the most diverse avian territories globally. Antioquia ranks among the top four provinces with the greatest variety of bird species, underscoring the importance of research efforts on the local bird fauna. Therefore, this study aimed to identify bacterial agents in the blood of wild birds from the municipality of Jardín (Antioquia, Colombia) by 16S qPCR sequencing. A descriptive cross-sectional study was conducted using non-probabilistic convenience sampling. Wild birds were captured using mist nets and blood samples were collected from each animal via puncture using sterile lancets in the brachial vein, and a drop of blood was collected on filter paper for qPCR analysis. The 16S gene in bacterial genomes was found in 13 out of 46 wild birds of the Passeriform and quasi-Passeriform orders, captured at three different locations within the study municipality at altitudes ranging from 1,665 to 2,034 m.a.s.l. Seven different bird species were recorded and four different haemobacteria were identified (i.e. Exiguobacterium spp., Escherichia coli, Stenotrophomonas spp., and Stenotrophomonas maltophilia). This study contributes to the knowledge in Colombia by identifying four different hemobacteria in wild birds. Further research is required on the health status of these birds and the attributable impacts on their populations and other related factors, including humans.

Downloads

Download data is not yet available.

References

  1. Adegoke, A. A., Stenström, T. A., & Okoh, A. I. (2017). Stenotrophomonas maltophilia as an emerging ubiquitous pathogen: Looking beyond contemporary antibiotic therapy. Frontiers in Microbiology, 8, 2276. https://doi.org/10.3389/fmicb.2017.02276
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410. https://doi.org/10.1016/S0022-2836(05)80360-2
  3. Álvarez, M., Córdoba-Córdoba, S., Escobar, F., Fagua, G., Gast, F., Medoza, H., Ospina, M., Umaña, A. M., & Villareal, H. (2004). Manual de métodos para el desarrollo de inventarios de biodiversidad. Programa de inventarios de biodiversidad. Instituto de investigación de recursos biológicos Alexander von Humboldt. http://hdl.handle.net/20.500.11761/31419
  4. Amery-Gale, J., Legione, A. R., Marenda, M. S., Owens, J., Eden, P. A., Konsak-Ilievski, B. M., Whiteley, P. L., Dobson, E. C., Browne, E. A., Slocombe, R. F., & Devlin, J. M. (2020). Surveillance for Chlamydia spp. with multilocus sequence typing analysis in wild and captive birds in Victoria, Australia. Journal of Wildlife Diseases, 56(1), 16–26.
  5. Bélanger, L., Garenaux, A., Harel, J., Boulianne, M., Nadeau, E., & Dozois, C. M. (2011). Escherichia coli from animal reservoirs as a potential source of human extraintestinal pathogenic E. coli. FEMS Immunology and Medical Microbiology, 62(1), 1–10. https://doi.org/10.1111/j.1574-695X.2011.00797.x
  6. Borges, C. A., Cardozo, M. V., Beraldo, L. G., Oliveira, E. S., Maluta, R. P., Barboza, K. B., Werther, K., & Ávila, F. A. (2017). Wild birds and urban pigeons as reservoirs for diarrheagenic Escherichia coli with zoonotic potential. Journal of Microbiology (Seoul, Korea), 55(5), 344–348. https://doi.org/10.1007/s12275-017-6523-3
  7. Brooke, J. S. (2012). Stenotrophomonas maltophilia: an emerging global opportunistic pathogen. Clinical microbiology reviews, 25(1), 2–41. https://doi.org/10.1128/CMR.00019-11
  8. Brooke J. S. (2021). Advances in the microbiology of Stenotrophomonas maltophilia. Clinical microbiology reviews, 34(3), e0003019. https://doi.org/10.1128/CMR.00030-19
  9. Calderón-Toledo, C., von Mentzer, A., Agramont, J., Thorell, K., Zhou, Y., Szabó, M., Colque, P., Kuhn, I., Gutiérrez-Cortez, S., & Joffré, E. (2023). Circulation of enterotoxigenic Escherichia coli (ETEC) isolates expressing CS23 from the environment to clinical settings. bioRxiv. https://doi.org/10.1101/2023.02.08.527665
  10. Cárdenas-Arias, A., Sano, E., Cardoso, B., Fuga, B., Sellera, F. P., Esposito, F., Aravena-Ramírez, V., Carhuaricra Huamán, D., Duran Gonzales, C., Luna Espinoza, L., Maturrano Hernández, L., & Lincopan, N. (2023). Genomic data of global clones of CTX-M-65-producing Escherichia coli ST10 from South American llamas inhabiting the Andean highlands of Peru. Journal of Global Antimicrobial Resistance. https://doi.org/10.1016/j.jgar.2023.11.011
  11. Chan, J. F., To, K. K., Tse, H., Jin, D. Y., & Yuen, K. Y. (2013). Interspecies transmission and emergence of novel viruses: lessons from bats and birds. Trends in Microbiology, 21(10), 544–555. https://doi.org/10.1016/j.tim.2013.05.005
  12. Dos Santos, E. J. E., Azevedo, R. P., Lopes, A. T. S., Rocha, J. M., Albuquerque, G. R., Wenceslau, A. A., Miranda, F. R., Rodrigues, D. D. P., & Maciel, B. M. (2020). Salmonella spp. in wild free-living birds from atlantic forest fragments in Southern bahia, Brazil. BioMed Research International, 2020, 7594136. https://doi.org/10.1155/2020/7594136
  13. El-Demerdash, A. S., Mowafy, R. E., Fahmy, H. A., Matter, A. A., & Samir, M. (2023). Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt. World Journal of Microbiology & Biotechnology, 39(12), 335. https://doi.org/10.1007/s11274-023-03774-2
  14. Farrow, J. A., Wallbanks, S., & Collins, M. D. (1994). Phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera Caryophanon, Exiguobacterium, Kurthia, and Planococcus. International Journal of Systematic Bacteriology, 44(1), 74–82. https://doi.org/10.1099/00207713-44-1-74
  15. Fuentes-Castillo, D., Esposito, F., Cardoso, B., Dalazen, G. T., Moura, Q., Fuga, B., Fontana, H., Cerdeira, L., Dropa, M., Rottmann, J., González-Acuña, D., Catão-Dias, J. L., & Lincopan, N. (2020). Genomic data reveal international lineages of critical priority Escherichia coli harbouring wide resistome in Andean condors (Vultur gryphus Linnaeus, 1758). Molecular Ecology. https://doi.org/10.1111/MEC.15455
  16. Gaukler, S. M., Linz, G. M., Sherwood, J. S., Dyer, N. W., Bleier, W. J., Wannemuehler, Y. M., Nolan, L. K., & Logue, C. M. (2009). Escherichia coli, Salmonella, and Mycobacterium avium subsp. paratuberculosis in wild European starlings at a Kansas cattle feedlot. Avian Diseases, 53(4), 544–551. https://doi.org/10.1637/8920-050809-Reg.1
  17. Hall, T. A. (1999). Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series, 4, 95–98.
  18. Hashish, A., Johnson, T. J., Chundru, D., Williams, M. L., Sato, Y., Macedo, N. R., Clessin, A., Gantelet, H., Bost, C., Tornos, J., Gamble, A., LeCount, K. J., Ghanem, M., Boulinier, T., & El-Gazzar, M. (2023). Complete genome sequences of two Pasteurella multocida isolates from seabirds. Microbiology Resource Announcements, 12(4), e0136522. https://doi.org/10.1128/mra.01365-22
  19. Higgins, D. G., Bleasby, A. J., & Fuchs, R. (1992). CLUSTAL V: improved software for multiple sequence alignment. Computer applications in the biosciences: CABIOS, 8(2), 189–191. https://doi.org/10.1093/bioinformatics/8.2.189
  20. Hilty, L. S., & Brown, L. W. (2021). Birds of Colombia. Lynx Editions.
  21. Hollis, D. G., & Weaver, R. E. (1981). Gram-positive organisms: a guide to identification. Special Bacteriology Section. Atlanta Centers for Disease Control.
  22. IUCN. The IUCN red list of threatened species. Version 2023-1. IUCN Red List of Threatened Species 2023. https://www.iucnredlist.org/
  23. Knöbl, T., Saidenberg, A. B. S., Moreno, A. M., Gomes, T. A. T., Vieira, M. A. M., Leite, D. S., Blanco, J. E., & Ferreira A. J. P. (2011). Serogroups and virulence genes of Escherichia coli isolated from psittacine birds. Pesquisa Veterinaria Brasileira, 31, 916–921. https://doi.org/10.1590/S0100-736X2011001000013
  24. Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis Across Computing Platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096
  25. Lagerstrom, K. M., & Hadly, E. A. (2023). Under-appreciated phylogroup diversity of Escherichia coli within and between animals at the urban-wildland interface. Applied and Environmental Microbiology, 89(6), e0014223. https://doi.org/10.1128/aem.00142-23
  26. Levin, II., & Parker, P. G. (2012). Haemosporidian parasites: Impacts on avian hosts. In: Miller RE, Murray F editors. Fowler’s Zoo and Wild Animal Medicine. https://doi.org/10.1016/b978-1-4377-1986-4.00047-0
  27. Li, Y., Liu, X., Chen, L., Shen, X., Wang, H., Guo, R., Li, X., Yu, Z., Zhang, X., Zhou, Y., & Fu, L. (2023). Comparative genomics analysis of Stenotrophomonas maltophilia strains from a community. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2023.1266295
  28. Liu, S. Y., Li, K. P., Hsieh, M. K., Chang, P. C., Shien, J. H., & Ou, S. C. (2019). Prevalence and genotyping of Chlamydia psittaci from domestic waterfowl, companion birds, and wild birds in Taiwan. Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 19(9), 666–673. https://doi.org/10.1089/vbz.2018.2403
  29. Mackenzie, J. S., & Jeggo, M. (2019). The One Health approach-Why is it so important? Tropical medicine and infectious disease, 4(2), 88. https://doi.org/10.3390/tropicalmed4020088
  30. Mukerji, S., Gunasekera, S., Dunlop, J. N., Stegger, M., Jordan, D., Laird, T., Abraham, R. J., Barton, M., O'Dea, M., & Abraham, S. (2020). Implications of foraging and interspecies interactions of birds for carriage of Escherichia coli strains resistant to critically important antimicrobials. Applied and Environmental Microbiology, 86(20), e01610-20. https://doi.org/10.1128/AEM.01610-20
  31. Murphy, R., Palm, M., Mustonen, V., Warringer, J., Farewell, A., Parts, L., & Moradigaravand, D. (2021). Genomic epidemiology and evolution of Escherichia coli in wild animals in Mexico. mSphere, 6(1), e00738-20. https://doi.org/10.1128/mSphere.00738-20
  32. Naqvi, M. A., Khan, M. K., Iqbal, Z., Rizwan, H. M., Khan, M. N., Naqvi, S. Z., Zafar, A., Sindhu, Z. U. D., Abbas, R. Z., & Abbas, A. (2017). Prevalence and associated risk factors of haemoparasites, and their effects on hematological profile in domesticated chickens in District Layyah, Punjab, Pakistan. Preventive Veterinary Medicine, 143, 49–53. https://doi.org/10.1016/j.prevetmed.2017.05.001
  33. Pennycott, T. W., Park, A., & Mather, H. A. (2006). Isolation of different serovars of Salmonella enterica from wild birds in Great Britain between 1995 and 2003. The Veterinary Record, 158(24), 817–820. https://doi.org/10.1136/vr.158.24.817
  34. Pérez-Rodríguez, A., de la Hera, I., Fernández-González, S., & Pérez-Tris, J. (2014). Global warming will reshuffle the areas of high prevalence and richness of three genera of avian blood parasites. Global Change Biology, 20(8), 2406–2416. https://doi.org/10.1111/gcb.12542
  35. Pfeiffer, I., Burger, J., & Brenig, B. (2004). Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle, sheep, goat, roe buck and deer by PCR-RFLP. BMC Genetics, 5, 30. https://doi.org/10.1186/1471-2156-5-30
  36. Ralph, C. J., Geupel, G. R., Pyle, P., Martin, T. E., DeSante, D. F., & Milá, B. (1996). Manual de métodos de campo para el monitoreo de aves terrestres. General Technical Reports, PSW-GTR-159. Albany, CA: US Department of Agriculture, Forest Service, Pacific Southwest Research Station. https://doi.org/10.2737/PSW-GTR-159
  37. Remsen, J., Areta, J., Cadena, C. D., Claramunt, S., Jaramillo, A., Pacheco, J. F., Pérez-Emán, J., Robbins, M. B., Stiles, F. G., Stotz, D. F., & Zimmer, K. J. (2022). A classification of the bird species of South America. American Ornithologists’. American Ornithological Society. https://www.museum.lsu.edu/~Remsen/SACCBaseline.htm
  38. Rybak, B., Krawczyk, B., Furmanek-Blaszk, B., Wysocka, M., Fordon, M., Ziolkowski, P., Meissner, W., Stepniewska, K., & Sikorska, K. (2022). Antibiotic resistance, virulence, and phylogenetic analysis of Escherichia coli strains isolated from free-living birds in human habitats. PloS One, 17(1), e0262236. https://doi.org/10.1371/journal.pone.0262236
  39. Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4), 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
  40. Stokes, H. S., Martens, J. M., Walder, K., Segal, Y., Berg, M. L., & Bennett, A. T. D. (2020). Species, sex and geographic variation in chlamydial prevalence in abundant wild Australian parrots. Scientific Reports, 10(1), 20478. https://doi.org/10.1038/s41598-020-77500-5
  41. Tamura, K., & Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10(3), 512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
  42. Toledano, P., Gomez, A. C., López, M., Alonso, C. A., Ruiz, J., Lagos, J., & Sáenz, Y. (2023). Phenotypic characteristics and clonal relationships of *Stenotrophomonas maltophilia* isolates in hospitalized adults from a private center in Lima, Peru. *Infection and Chemotherapy*. https://doi.org/10.3947/ic.2023.0003
  43. Vélez, D., Tamayo, E., Ayerbe-Quiñones, F., Torres, J., Rey, J., Castro-Moreno, C., Ramírez, B., & Ochoa-Quintero, J. M. (2021). Distribution of birds in Colombia. Biodiversity Data Journal, 9, e59202. https://doi.org/10.3897/BDJ.9.e59202
  44. Vishnivetskaya, T. A., Kathariou, S., & Tiedje, J. M. (2009). The Exiguobacterium genus: biodiversity and biogeography. Extremophiles: Life Under Extreme Conditions, 13(3), 541–555. https://doi.org/10.1007/s00792-009-0243-5
  45. Xu, Y., Zhang, Z., Su, Z., Zhou, X., Han, X., & Liu, Q. (2020). Continuous microfluidic purification of DNA using magnetophoresis. Micromachines, 11(2), 187. https://doi.org/10.3390/mi11020187
  46. Xu, Y., Cheng, T., Rao, Q., Zhang, S., & Ma, Y. (2023). Comparative genomic analysis of Stenotrophomonas maltophilia unravels their genetic variations and versatility trait. Journal of Applied Genetics. https://doi.org/10.1007/s13353-023-00752-0

Make a Submission

Make a Submission

Journal Metrics (2023) & Ranking

Impact Factor
→
0.5 (2024)
5 years Impact Factor
→
0.8
JCR Quartile
→
Q4
JIF Rank
→
134/170 (Veterinary Sciences)
SJR (2024)
→
0.244
SNIP (2024)
→
0.35

 

 

SCImago Journal & Country Rank

Indexed in

Publisher


This journal is published by the Facultad de Ciencias Veterinarias, Universidad Austral de Chile

Keywords