Skip to main content Skip to main navigation menu Skip to site footer
Short communication
Austral J. Vet. Sci.
Vol 51, 119-123 (2019)
DOI: 10.4067/S0719-81322019000300119

Cryopreservation of horse semen with a liposome and trehalose added extender

1 Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, México.
2 Departamento de Reproducción Animal, Facultad de Veterinaria, Universidad de la República de Uruguay, Montevideo, Uruguay.
Keywords: equine semen liposomes trehalose

Submitted: 2018-05-24

Accepted: 2019-01-03

Published: 2019-09-01

*Corresponding author:
mbarrientos@uv.mx

How to Cite

Medina-León, A. Z., Domínguez-Mancera, B., Cazalez-Penino, N., Cervantes-Acosta, P., Jácome-Sosa, E., Romero-Salas, D., & Barrientos-Morales, M. (2019). Cryopreservation of horse semen with a liposome and trehalose added extender. Austral Journal of Veterinary Sciences, 51(3), 119–123. https://doi.org/10.4067/S0719-81322019000300119

License

Copyright (c) 2019 Ariadna Z. Medina-León, Belisario Domínguez-Mancera, Nicolás Cazalez-Penino, Patricia Cervantes-Acosta, Edelmira Jácome-Sosa, Dora Romero-Salas, Manuel Barrientos-Morales

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The aim of the study was to evaluate the effect of cryopreserved equine semen in the presence of trehalose-loaded liposomes on the integrity and function of sperm cells. Six ejaculations of five stallions collected with an artificial vagina were used. The ejaculates were diluted with INRA 96® 2:1 v/v and transported at 22 °C to the laboratory. Before cryopreservation, the semen was diluted with INRA Freeze® to obtain the following treatments: T1) INRA Freeze® (control), T2) INRA Freeze® + liposomes, T3) INRA Freeze® + liposomes+trehalose. Data were analysed using the Kruskal Wallis test. The percentages of sperm with intact DNA were 54.5, 57.9, and 64.8% for T1, T2 and T3, respectively (P > 0.05). When evaluating the acrosomal and capacitation state after filtering with Percoll®, the percentages of spermatozoa without acrosome reaction and without capacitation were 67.8, 79.2 and 68.1% in T1, T2, and T3, respectively (P > 0.05), while the capacitated sperm without acrosome reaction and without capacitation was similar in T1 (47%) and T3 (32%) (P > 0.05), and lower in T2 (16%) before filtering with Percoll®. The use of liposomes and liposome-trehalose did not affect on the functional status and nuclear chromatin of the equine sperm after freezing, but it did affect the percentage of capacitated sperm without acrosome reaction after selecting the thawed semen using the Percoll® gradient.

Downloads

Download data is not yet available.

References

  1. Alvarenga MA, Ozanam F, Ramires C. 2015. Cryopreservation of Stallion Semen. In: KA Sprayberry, NE Robinson (eds). Robinson’s Current Therapy in Equine Medicine. 7th ed. Saunders Elsevier, St Louis, Mo, USA, Pp 663-664.
  2. Amann R, Pickett B. 1987. Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. J Equine Vet Science 7, 145-173.
  3. Ansari MS, Rakha BA, Akhter S, Ashiq M. 2016. OptiXcell improves the postthaw quality and fertility of buffalo bull sperm. Theriogenology 85, 528-532.
  4. Belala R, Delay J, Amirat L, Ropers MH, Guillou JL, et al. 2016. The benefits of liposomes for chilling canine sperm for 4 days at 4 °C. Ani Reprod Sci 168, 100-109.
  5. Brito L, Althouse G, Aurich C, Chenoweth P, Eilts B, et al. 2016. Andrology laboratory review: Evaluation of sperm concentration. Theriogenology 85, 1507-1527.
  6. Bucak M, Atessahin A, Varıs L, Yuce A, Tekin N, et al. 2007. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: microscopic and oxidative stress parameters after the freeze-thawing process. Theriogenology 67, 1060-1067.
  7. Corcuera B, Marigorta P, Sagüés A, Saiz-Cidoncha F, Pérez-Gutiérrez JF. 2007. Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa. Theriogenology 67, 1150-1157.
  8. Das Gupta S, Mills S, Fraser L. 1994. A possible role for Ca2+-ATPase in human sperm capacitation. J Reprod Fertil 102, 107-116.
  9. De Leeuw F, De Leeuw A, Dendaas J, Colenbrander B, Verkleij A. 1993. Effects of various cryoprotective agents and membrane- stabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology 30, 32-44.
  10. Denniston DJ, Graham JK, Squires EL, Brinsko SP. 1997. The effect of liposomes composed of phosphatidylserine and cholesterol on fertility rates using frozen thawed equine spermatozoa. J Equine Vet Sci 17, 675-676.
  11. Hamamah S, Royere D, Nicolle JC, Paquignon M, Lansac J. 1990. Effects of freezing-thawing on the spermatozoon nucleus: a comparative chromatin cytophotometric study in the porcine and human species. Reprod Nutr Dev 30, 59-64.
  12. Kheirolomoom A, Satpathy GR, Torok Z, Banerjee M, Bali R, et al. 2005. Phospholipid vesicles increase the survival of freeze-dried human red blood cells. Cryobiology 51, 290-305.
  13. Köse GT, Arica MY, Hasirci V. 1998. Low-molecular-weight heparin-conjugated liposomes with improved stability and hemocompatibility. Drug Delivery 5, 257-264.
  14. Kumar P, Sainia M, Kumar D, Balharaa A, Yadava S, et al. 2015. Liposome-based semen extender is suitable alternative to egg yolkbased extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim Reprod Sci 159, 38-45.
  15. Maxwell W, Long C, Johnson L, Dobrinsky L, Welch G. 1998. The relationship between membrane status and fertility of boar spermatozoa after flow cytometric sorting in the presence or absence of seminal plasma. Reprod Fertil Dev 10, 433-440.
  16. Molinia F, Evans G, Casares P, Maxwell W. 1994. Effect of monosaccharides and disaccharides in tris-based diluents on motility, acrosome integrity and fertility of pellet frozen ram spermatozoa. Anim Reprod Sci 36, 113-122.
  17. Moreno JMM, Harris ME, Breda J, Møller L, Sanchez JLA, et al. 2013. Enhanced labelling on alcoholic drinks: reviewing the evidence to guide alcohol policy. Eur J Public Health 23, 1082-1087.
  18. Mutalik S, Salian SR, Avadhani K, Menon J, Joshi H, et al. 2014. Liposome encapsulated soy lecithin and cholesterol can efficiently replace chicken egg yolk in human semen cryopreservation medium. Syst Biol Reprod Med 60, 183-188.
  19. Najjar A, Saïd SB, Kalamoun S, Benaoun B, Ezzaouia M, et al. 2016. Comparison of two freezing extenders and their influence on sperm quality of Tunisian Arab stallions. J Vet Sci Anim Husb 4, 302.
  20. Pillet E, Labbe C, Batellier F, Duchamp G, Beaumal V, et al. 2012. Liposomes as an alternative to egg yolk in stallion freezing extender. Theriogenology 77, 268-279.
  21. Quinn P, Chow P, White I. 1980. Evidence that phospholipid protects ram spermatozoa from cold shock at a plasma membrane site. J Reprod Fert 60, 403-407.
  22. Ramires NC, Monteiro GA, Sancler-Silva YFR, Papa P, Guasti P, et al. 2014. Comparison of different freezing extenders for semen cryopreservation from stallions with poor and good semen freezability. J Equine Vet Sci 34, 58-60.
  23. Röpke T, Oldenhof H, Leiding C, Sieme H, Bollwein H, et al. 2011. Liposomes for cryopreservation of bovine sperm. Theriogenology 76, 1465-1472.
  24. Tejada R, Mitchell C, Norman A, Marik J, Friedman S. 1984. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil steril 42, 87-91.
  25. Vera-Munoz O, Amirat-Briand L, Bencharif D, Anton M, Desherces S, et al. 2011. Effect of low-density lipoproteins, spermatozoa concentration and glycerol on functional and motility parameters of bull spermatozoa during storage at 4 °C. Asian J Androl 13, 281-286.
  26. Watson PF. 1981. The roles of lipid and protein in the protection of ram spermatozoa at 5 degrees C by egg-yolk lipoprotein. J Reprod Fertil 62, 483-492.
  27. Wilhelm K, Graham J, Squires E. 1996. Effects of phosphatidylserine and cholesterol liposomes on the viability, motility, and acrosomal integrity of stallion spermatozoa prior to and after cryopreservation. Cryobiology 33, 320-329.
  28. Wu T, Cheng F, Chen I, Yang C, Tsai C, et al. 2013. The combinatorial effect of different Equex STM paste concentrations, cryoprotectants and the straw-freezing methods on the post-thaw boar semen quality. Reprod Domest Anim 48, 53-58.

Make a Submission

Make a Submission

Journal Metrics (2023) & Ranking

Impact Factor
→
0.5 (2024)
5 years Impact Factor
→
0.8
JCR Quartile
→
Q4
JIF Rank
→
134/170 (Veterinary Sciences)
SJR (2024)
→
0.244
SNIP (2024)
→
0.35

 

 

SCImago Journal & Country Rank

Indexed in

Publisher


This journal is published by the Facultad de Ciencias Veterinarias, Universidad Austral de Chile

Keywords